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AIB1 / SRC-3 antibody (pAb)

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

ChIP, WB

Cat No : 39797,39798 39797

Synonyms

AIB1, Amplified in Breast Cancer 1, Steroid Receptor Coactivator-3, Nuclear Receptor Coactivator 3, SRC-3, NCOA3, HAT, co-activator, antibody, antibodies, polyclonal, sample

验证数据展示

  • AIB1 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit (Catalog No. 53008) and MCF-7 chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ul of AIB1 / SRC-3 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the pS2 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

    AIB1 antibody tested by ChIP analysis. Chromatin IP performed using the ChIP-IT Express Kit (Catalog No. 53008) and MCF-7 chromatin (1.5 x 106 cell equivalents per ChIP) using 10 ul of AIB1 / SRC-3 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using a primer pair specific for the pS2 gene promoter. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.

  • AIB1 antibody (pAb) tested by Western blot. Detection of AIB1 by Western blot. Lane 1: HeLa whole-cell extract (30 ug). Lane 2: Whole cell extract (30 ug) of MCF-7 cells. Both probed with AIB1 antibody at a 1:500 dilution.

    AIB1 antibody (pAb) tested by Western blot. Detection of AIB1 by Western blot. Lane 1: HeLa whole-cell extract (30 ug). Lane 2: Whole cell extract (30 ug) of MCF-7 cells. Both probed with AIB1 antibody at a 1:500 dilution.

产品信息

Tested Applications ChIP, WB

Applications Validated by Active Motif: ChIP: 10 ul per ChIP WB: 1:500 - 1:2,000 dilution
Tested Reactivity Human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen This AIB1 antibody was raised against a peptide derived from human AIB1/SRC-3.
Full Name AIB1 / SRC-3 antibody (pAb)
Synonyms AIB1, Amplified in Breast Cancer 1, Steroid Receptor Coactivator-3, Nuclear Receptor Coactivator 3, SRC-3, NCOA3, HAT, co-activator, antibody, antibodies, polyclonal, sample
Molecular weight 160 kDa
GenBank accession numberNP_858045
RRIDAB_2615066
Purification Method Affinity Purified
Buffer Purified IgG in 70 mM Tris (pH 8), 105 mM NaCl, 31 mM glycine, 0.07 mM EDTA, 30% glycerol and 0.035% sodium azide. Sodium azide is highly toxic.
Storage Some products may be shipped at room temperature. This will not affect their stability or performance. Avoid repeated freeze/thaw cycles by aliquoting items into single-use fractions for storage at -20°C for up to 2 years. Keep all reagents on ice when not in storage.

背景介绍

AIB1 (Amplified in Breast Cancer 1, Steroid Receptor Coactivator-3, Nuclear Receptor Coactivator 3, SRC-3, NCOA3) is a chromatin-associated protein that interacts with nuclear hormone receptors to modify their activity as transcriptional activators. AIB1 has histone acetyltransferase (HAT) activity and recruits p300/CBP-associated factor (PCAF) and CREB binding protein (CBP) as part of a multisubunit co-activator complex. This protein is initially found in the cytoplasm but is translocated into the nucleus upon phosphorylation. It is Involved in the coactivation of different nuclear receptors, such as GR, ER, RARs, RXRs, TRs, VDR and PPARs. It is also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFkB1 protein.