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AIFM2/ FSP1 Monoclonal antibody, PBS Only

AIFM2/ FSP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

mouse, human

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A2B2

Cat no : 68049-1-PBS

Print datasheet

Synonyms

AMID, AIFM2, FSP1, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, Apoptosis inducing factor 2

验证数据展示

  • WB analysis using 68049-1-Ig (same clone as 68049-1-PBS)

    WB analysis using 68049-1-Ig (same clone as 68049-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    WB analysis of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    IP experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    IP result of anti-AIFM2/ FSP1 (IP:68049-1-Ig, 4ug; Detection:68049-1-Ig 1:500) with K-562 cells lysate 1760 ug. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    FC experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human AIFM2 (68049-1-Ig, Clone:1A2B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • IF Staining of H9C2 using 68049-1-Ig (same clone as 68049-1-PBS)

    IF Staining of H9C2 using 68049-1-Ig (same clone as 68049-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using AIFM2/ FSP1 antibody (68049-1-Ig, Clone: 1A2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68049-1-Ig (AIFM2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AIFM2 antibody included
  • IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68049-1-Ig (AIFM2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AIFM2 antibody included
Planning an IHC experiment? We recommend our IHCeasy AIFM2 Ready-To-Use IHC Kit. AIFM2 primary antibody included.

产品信息

68049-1-PBS targets AIFM2/ FSP1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with mouse, human samples.

Tested Applications WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
Tested Reactivity mouse, human
Immunogen AIFM2/ FSP1 fusion protein Ag30516 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name apoptosis-inducing factor, mitochondrion-associated, 2
Synonyms AMID, AIFM2, FSP1, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, Apoptosis inducing factor 2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC023601
Gene Symbol AIFM2/ FSP1
Gene ID (NCBI) 84883
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BRQ8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.