• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 68049-1-Ig (same clone as 68049-1-PBS)

    WB analysis using 68049-1-Ig (same clone as 68049-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    WB analysis of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    IP experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    IP result of anti-AIFM2/ FSP1 (IP:68049-1-Ig, 4ug; Detection:68049-1-Ig 1:500) with K-562 cells lysate 1760 ug. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    FC experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.5 ug Anti-Human AIFM2 (68049-1-Ig, Clone:1A2B2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • IF Staining of H9C2 using 68049-1-Ig (same clone as 68049-1-PBS)

    IF Staining of H9C2 using 68049-1-Ig (same clone as 68049-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using AIFM2/ FSP1 antibody (68049-1-Ig, Clone: 1A2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68049-1-Ig (AIFM2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AIFM2 antibody included
  • IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68049-1-Ig (AIFM2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AIFM2 antibody included

AIFM2/ FSP1 Monoclonal antibody, PBS Only

AIFM2/ FSP1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 68049-1-PBS

产品说明书

CloneNo. 1A2B2

宿主/亚型

Mouse / IgG1

种属反应性

mouse, human

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

AMID, AIFM2, FSP1, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, Apoptosis inducing factor 2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy AIFM2 Ready-To-Use IHC Kit. AIFM2 primary antibody included.

产品信息

68049-1-PBS targets AIFM2/ FSP1 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with mouse, human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 mouse, human
免疫原 AIFM2/ FSP1 fusion protein Ag30516 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 apoptosis-inducing factor, mitochondrion-associated, 2
别名 AMID, AIFM2, FSP1, Apoptosis-inducing factor homologous mitochondrion-associated inducer of death, Apoptosis inducing factor 2
计算分子量 41 kDa
观测分子量41 kDa
GenBank蛋白编号BC023601
基因名称 AIFM2/ FSP1
Gene ID (NCBI) 84883
RRIDAB_2918791
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ9BRQ8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.