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AIFM2/ FSP1 Monoclonal antibody, PBS Only

AIFM2/ FSP1 Monoclonal Antibody for WB, IP, IF, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IP, IF, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1A2B2

Cat No : 68049-1-PBS

Print datasheet

Synonyms

AIFM2, AIFM2/ FSP1, AMID, Apoptosis inducing factor 2, FSP1, p53 responsive gene 3 protein, PRG3, RP11 367H5.2

验证数据展示

  • WB analysis using 68049-1-Ig (same clone as 68049-1-PBS)

    WB analysis using 68049-1-Ig (same clone as 68049-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    WB analysis of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 68049-1-Ig (AIFM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • IP experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    IP experiment of K-562 using 68049-1-Ig (same clone as 68049-1-PBS)

    IP result of anti-AIFM2/ FSP1 (IP:68049-1-Ig, 4ug; Detection:68049-1-Ig 1:500) with K-562 cells lysate 1760 ug. This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • IF Staining of H9C2 using 68049-1-Ig (same clone as 68049-1-PBS)

    IF Staining of H9C2 using 68049-1-Ig (same clone as 68049-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed H9C2 cells using AIFM2/ FSP1 antibody (68049-1-Ig, Clone: 1A2B2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68049-1-Ig (AIFM2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AIFM2 antibody included
  • IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    IHC staining of mouse liver using 68049-1-Ig (same clone as 68049-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68049-1-Ig (AIFM2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68049-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AIFM2 antibody included
Planning an IHC experiment? We recommend our IHCeasy AIFM2 Ready-To-Use IHC Kit. AIFM2 primary antibody included.

产品信息

68049-1-PBS targets AIFM2/ FSP1 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IP, IF, IHC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse
Immunogen AIFM2/ FSP1 fusion protein Ag30516 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name apoptosis-inducing factor, mitochondrion-associated, 2
Synonyms AIFM2, AIFM2/ FSP1, AMID, Apoptosis inducing factor 2, FSP1, p53 responsive gene 3 protein, PRG3, RP11 367H5.2
Calculated Molecular Weight 41 kDa
Observed Molecular Weight41 kDa
GenBank Accession NumberBC023601
Gene Symbol AIFM2
Gene ID (NCBI) 84883
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ9BRQ8
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

The human AIFM2 protein (also known as FSP1 or AMID) is an apoptosis associated flavoprotein with a 6-hydroxy FAD cofactor. AIFM2 is a NAD(P)H-binding oxidoreductase with some sequence similarities to A1FM1 (formerly known as AIF, Apoptosis Inducing Factor), a mitochondrion-associated enzyme which relocates to the cell nucleus during apoptosis and is considered to be a key player in the progression of cell death.