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验证数据展示

  • WB analysis using 14778-1-AP

    WB analysis using 14778-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14778-1-AP (AIFM3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of human ovary tumor using 14778-1-AP

    IHC staining of human ovary tumor using 14778-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14778-1-AP (AIFM3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human ovary tumor using 14778-1-AP

    IHC staining of human ovary tumor using 14778-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 14778-1-AP (AIFM3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 14778-1-AP

    IHC staining of human colon cancer using 14778-1-AP

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 14778-1-AP (AIFM3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human colon cancer using 14778-1-AP

    IHC staining of human colon cancer using 14778-1-AP

    Immunohistochemical analysis of paraffin-embedded Colorectal cancer slide using 14778-1-AP (AIFM3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AIFM3 Polyclonal antibody

AIFM3 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 14778-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, ELISA

别名

EC:1.-.-.-, Apoptosis-inducing factor-like protein, Apoptosis-inducing factor 3, Apoptosis inducing factor 3, AIFL

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, U-87 MG cells
Positive IHC detected inhuman ovary tumor tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14778-1-AP targets AIFM3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AIFM3 fusion protein Ag6278 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 apoptosis-inducing factor, mitochondrion-associated, 3
别名 EC:1.-.-.-, Apoptosis-inducing factor-like protein, Apoptosis-inducing factor 3, Apoptosis inducing factor 3, AIFL
计算分子量 67 kDa
观测分子量 67 kDa
GenBank蛋白编号BC032485
基因名称 AIFM3
Gene ID (NCBI) 150209
RRIDAB_2258090
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96NN9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Apoptosis-inducing factor, mitochondrion-associated 3 (AIFM3) is a mitochondrial protein/flavoenzyme and its gene is located on chromosome 22q11.21. Mature human AIFM3 protein consists of 598 amino acids with a molecular weight of 66 kDa and is predominantly localized in the mitochondria. AIFM3 is widely expressed in many tissues, and breast cancer tissues and cholangiocarcinoma tissue (PMID: 32664187).

实验方案

Product Specific Protocols
WB protocol for AIFM3 antibody 14778-1-APDownload protocol
IHC protocol for AIFM3 antibody 14778-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols