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AIG1 Polyclonal antibody

AIG1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14468-1-AP

Print datasheet

Synonyms

AIG 1, AIG1, androgen induced 1

验证数据展示

  • IHC staining of human ovary tumor using 14468-1-AP

    IHC staining of human ovary tumor using 14468-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 14468-1-AP (AIG1 antibody) at dilution of 1:100 (under 40x lens).

  • WB analysis using 14468-1-AP

    WB analysis using 14468-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14468-1-AP (AIG1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inK-562 cells, mouse heart tissue, mouse testis tissue, PC-3 cells, rat heart tissue, rat ovary tissue, mouse ovary tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14468-1-AP targets AIG1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AIG1 fusion protein Ag5836 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name androgen-induced 1
Synonyms AIG 1, AIG1, androgen induced 1
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28-32 kDa
GenBank Accession NumberBC025278
Gene Symbol AIG1
Gene ID (NCBI) 51390
RRIDAB_2273794
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NVV5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AIG1 antibody 14468-1-APDownload protocol
IHC protocol for AIG1 antibody 14468-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols