- Featured Product
- KD/KO Validated
AIM2 Polyclonal antibody
AIM2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (1)
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HeLa cells, Jurkat cells, SH-SY5Y cells, mouse testis tissue, rat testis tissue, MCF-7 cells, A549 cells, COLO 320 cells, HepG2 cells, Raji cells |
Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:1000-1:6000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
20590-1-AP targets AIM2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, ELISA Application Description |
Cited Applications | WB, IHC |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, zebrafish |
Immunogen | AIM2 fusion protein Ag14520 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | absent in melanoma 2 |
Synonyms | PYHIN4, Interferon-inducible protein AIM2 |
Calculated Molecular Weight | 343 aa, 39 kDa |
Observed Molecular Weight | 39-42 kDa |
GenBank Accession Number | BC010940 |
Gene Symbol | AIM2 |
Gene ID (NCBI) | 9447 |
RRID | AB_10694420 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | O14862 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for AIM2 antibody 20590-1-AP | Download protocol |
IHC protocol for AIM2 antibody 20590-1-AP | Download protocol |
IF protocol for AIM2 antibody 20590-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Cell Mol Immunol NLRP3-dependent pyroptosis is required for HIV-1 gp120-induced neuropathology.
| ||
Mucosal Immunol AIM2 contributes to the maintenance of intestinal integrity via Akt and protects against Salmonella mucosal infection. | ||
Front Immunol Cytokine Secretion and Pyroptosis of Thyroid Follicular Cells Mediated by Enhanced NLRP3, NLRP1, NLRC4, and AIM2 Inflammasomes Are Associated With Autoimmune Thyroiditis. | ||
Front Cell Dev Biol Autophagy-Based Unconventional Secretory for AIM2 Inflammasome Drives DNA Damage Resistance During Intervertebral Disc Degeneration.
| ||
Front Immunol Crosstalk of necroptosis and pyroptosis defines tumor microenvironment characterization and predicts prognosis in clear cell renal carcinoma | ||
Front Oncol Identification of pyroptosis-related lncRNA signature and AC005253.1 as a pyroptosis-related oncogene in prostate cancer |