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AIM2 Monoclonal antibody, PBS Only

AIM2 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, rabbit

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3E12A8

Cat No : 66902-1-PBS

Print datasheet

Synonyms

absent in melanoma 2, AIM2, PYHIN4

验证数据展示

  • WB analysis using 66902-1-Ig (same clone as 66902-1-PBS)

    WB analysis using 66902-1-Ig (same clone as 66902-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66902-1-Ig (AIM2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

  • IHC staining of human urothelial carcinoma using 66902-1-Ig (same clone as 66902-1-PBS)

    IHC staining of human urothelial carcinoma using 66902-1-Ig (same clone as 66902-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 66902-1-Ig (AIM2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66902-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AIM2 antibody included
Planning an IHC experiment? We recommend our IHCeasy AIM2 Ready-To-Use IHC Kit. AIM2 primary antibody included.

产品信息

66902-1-PBS targets AIM2 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat, rabbit samples.

Tested Applications WB, IHC, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat, rabbit
Immunogen AIM2 fusion protein Ag14607 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name absent in melanoma 2
Synonyms absent in melanoma 2, AIM2, PYHIN4
Calculated Molecular Weight 343 aa, 39 kDa
Observed Molecular Weight 37-39 and 53 kDa
GenBank Accession NumberBC010940
Gene Symbol AIM2
Gene ID (NCBI) 9447
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO14862
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Absent in melanoma 2 (AIM2) is a sensor of cytosolic DNA that is responsible for activation of the inflammasome and host immune responses to DNA viruses and intracellular bacteria [PMID: 22695634]. Upon binding to DNA, it is thought to undergo oligomerization and to associate with PYCARD initiating the recruitment of caspase-1 precusrsor and processing of interleukin-1 beta and interleukin-18. Otherwise, it functions as a tumor suppressor which may act by repressing NF-kappa-B transcriptional activity [PMID:16432157,20566831]. 66902-1-Ig antibody detects the 37-39 and 53 kDa protein in SDS-PAGE similar to the paper published. [PMID: 28809949]