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AIP/ARA9 Polyclonal antibody

AIP/ARA9 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA and More (1)

Conjugate

Unconjugated

Cat no : 18176-1-AP

Print datasheet

Synonyms

ARA9, AIP, XAP 2, XAP2, FKBP37

验证数据展示

  • WB analysis of HepG2 using 18176-1-AP

    WB analysis of HepG2 using 18176-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 18176-1-AP

    IHC staining of human liver cancer using 18176-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with AIP antibody included
  • IHC staining of human liver cancer using 18176-1-AP

    IHC staining of human liver cancer using 18176-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18176-1-AP (AIP/ARA9 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with AIP antibody included
  • FC experiment of HeLa using 18176-1-AP

    FC experiment of HeLa using 18176-1-AP

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug AIP/ARA9 Polyclonal antibody (18176-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHepG2 cells, HeLa cells, NIH/3T3 cells
Positive IHC detected inhuman liver cancer tissue, human heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy AIP Ready-To-Use IHC Kit. AIP primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18176-1-AP targets AIP/ARA9 in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, FC (Intra), ELISA Application Description
Cited ApplicationsWB, IHC, IP
Tested Reactivity human, mouse, rat
Cited Reactivitymouse, rat
Immunogen AIP/ARA9 fusion protein Ag12869 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aryl hydrocarbon receptor interacting protein
Synonyms ARA9, AIP, XAP 2, XAP2, FKBP37
Calculated Molecular Weight 330 aa, 38 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC104797
Gene Symbol AIP
Gene ID (NCBI) 9049
RRIDAB_11182727
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO00170
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AIP/ARA9 antibody 18176-1-APDownload protocol
IHC protocol for AIP/ARA9 antibody 18176-1-APDownload protocol
FC protocol for AIP/ARA9 antibody 18176-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseIP

J Clin Invest

Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy

Authors - Frank L. van de Veerdonk
View Article
ratWB,IHC

Heliyon

PSO/SDF-1 composite hydrogel promotes osteogenic differentiation of PDLSCs and bone regeneration in periodontitis rats

Authors - Wei Zhang
View Article