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验证数据展示

  • IP experiment of Y79 using 15108-1-AP

    IP experiment of Y79 using 15108-1-AP

    IP result of anti-AIPL1 (IP:15108-1-AP, 4ug; Detection:15108-1-AP 1:500) with Y79 cells lysate 2400 ug.

  • IHC staining of human eye using 15108-1-AP

    IHC staining of human eye using 15108-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 15108-1-AP (AIPL1 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human eye using 15108-1-AP

    IHC staining of human eye using 15108-1-AP

    Immunohistochemical analysis of paraffin-embedded human eye using 15108-1-AP (AIPL1 antibody) at dilution of 1:50 (under 40x lens).

  • WB analysis of Y79 using 15108-1-AP

    WB analysis of Y79 using 15108-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 15108-1-AP (AIPL1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

AIPL1 Polyclonal antibody

AIPL1 Polyclonal Antibody for WB, IP, IHC, ELISA
Cat No. 15108-1-AP

产品说明书

引用文献(2)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IP, IHC, ELISA and More (1)

别名

AIPL1, AIPL2, LCA4

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inY79 cells, mouse brain tissue, SH-SY5Y cells
Positive IP detected inY79 cells
Positive IHC detected inhuman eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15108-1-AP targets AIPL1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IP, IHC, ELISA Application Description
文献引用应用WB, IP, IF
经测试反应性 human, mouse, rat
文献引用反应性mouse
免疫原 AIPL1 fusion protein Ag4625 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aryl hydrocarbon receptor interacting protein-like 1
别名 AIPL1, AIPL2, LCA4
计算分子量 44 kDa
观测分子量 44 kDa
GenBank蛋白编号BC012055
基因名称 AIPL1
Gene ID (NCBI) 23746
RRIDAB_2224995
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZN9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AIPL1 antibody 15108-1-APDownload protocol
IHC protocol for AIPL1 antibody 15108-1-APDownload protocol
IP protocol for AIPL1 antibody 15108-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB,IF

Sci Rep

ZP3 is Required for Germinal Vesicle Breakdown in Mouse Oocyte Meiosis.

Authors - Lei-Lei Gao
View Article
mouseIP

Zygote

ZP3 and AIPL1 participate in GVBD of mouse oocytes by affecting the nuclear membrane localization and maturation of farnesylated prelamin A

Authors - Zhen Jin
View Article