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  • Featured Product
  • KD/KO Validated

AK2 Monoclonal antibody, PBS Only

AK2 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat, mouse

Applications

WB,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

3H2C10

Cat No : 66127-1-PBS

Print datasheet

Synonyms

adenylate kinase 2, ADK2, AK 2, AK2, ATP AMP transphosphorylase 2

验证数据展示

  • WB analysis using 66127-1-Ig (same clone as 66127-1-PBS)

    WB analysis using 66127-1-Ig (same clone as 66127-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66127-1-Ig (AK2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 66127-1-Ig (same clone as 66127-1-PBS)

    IHC staining of human liver using 66127-1-Ig (same clone as 66127-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AK2 antibody included
  • IHC staining of human liver using 66127-1-Ig (same clone as 66127-1-PBS)

    IHC staining of human liver using 66127-1-Ig (same clone as 66127-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66127-1-Ig (AK2 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AK2 antibody included
  • IF Staining of HepG2 using 66127-1-Ig (same clone as 66127-1-PBS)

    IF Staining of HepG2 using 66127-1-Ig (same clone as 66127-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 66127-1-Ig (AK2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 66127-1-Ig (same clone as 66127-1-PBS)

    FC experiment of HepG2 using 66127-1-Ig (same clone as 66127-1-PBS)

    1X10^6 HepG2 cells were stained with .2ug AK2 antibody (66127-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 66127-1-Ig (same clone as 66127-1-PBS)

    WB analysis of HeLa using 66127-1-Ig (same clone as 66127-1-PBS)

    WB result of AK2 antibody (66127-1-Ig; 1:60000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AK2 transfected HeLa cells. This data was developed using the same antibody clone with 66127-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy AK2 Ready-To-Use IHC Kit. AK2 primary antibody included.

产品信息

66127-1-PBS targets AK2 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human, rat, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF,FC Application Description
Tested Reactivity human, rat, mouse
Immunogen AK2 fusion protein Ag17911 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name adenylate kinase 2
Synonyms adenylate kinase 2, ADK2, AK 2, AK2, ATP AMP transphosphorylase 2
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 29 kDa
GenBank Accession NumberBC009405
Gene Symbol AK2
Gene ID (NCBI) 204
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP54819
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.