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验证数据展示

  • WB analysis using 85406-3-RR (same clone as 85406-3-PBS)

    WB analysis using 85406-3-RR (same clone as 85406-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85406-3-RR (AK2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85406-3-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP01927-3

    Sandwich ELISA standard curve of MP01927-3

    Sandwich ELISA standard curve of MP01927-3, Human AK2 Recombinant Matched Antibody Pair - PBS only. 85406-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1464. 85406-2-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL.

  • Sample test of MP01927-3

    Sample test of MP01927-3

    The mean AK2 concentration was determined to be 6.31 ng/mL in HeLa cell extract based on a 2.10 mg/mL extract load and 5.60 ng/mL in HL-60 cell extract based on a 1.50 mg/mL extract load.

  • Cytometric bead array standard curve of MP01927-1

    Cytometric bead array standard curve of MP01927-1

    Cytometric bead array standard curve of MP01927-1, AK2 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 85406-3-PBS. Detection antibody: 85406-1-PBS. Standard: Ag1464. Range: 0.781-100 ng/mL.

  • Affinity and Kinetic Characterization of 85406-3-RR

    Affinity and Kinetic Characterization of 85406-3-RR

    Biolayer interferometry (BLl) kinetic assays of 85406-3-RR against Human AK2 were performed. The affinity constant is 0.428 nM.

AK2 Recombinant antibody, PBS Only (Capture)

AK2 Uni-rAbTM Recombinant Antibody for WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 85406-3-PBS

产品说明书

CloneNo. 242899H7

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

别名

242899H7, adenylate kinase 2, Adenylate kinase 2, mitochondrial, N-terminally processed, Adenylate monophosphate kinase, ADK2

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy AK2 Ready-To-Use IHC Kit. AK2 primary antibody included.

产品信息

85406-3-PBS targets AK2 as part of a matched antibody pair:

MP01927-1: 85406-3-PBS capture and 85406-1-PBS detection (validated in Cytometric bead array)

MP01927-3: 85406-3-PBS capture and 85406-2-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human, mouse, rat
免疫原 AK2 fusion protein Ag1464 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 adenylate kinase 2
别名 242899H7, adenylate kinase 2, Adenylate kinase 2, mitochondrial, N-terminally processed, Adenylate monophosphate kinase, ADK2
计算分子量 26 kDa
观测分子量 26 kDa
GenBank蛋白编号BC009405
基因名称 AK2
Gene ID (NCBI) 204
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP54819
储存缓冲液 PBS Only
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AK2(Adenylate kinase 2, mitochondrial ) is also named as ADK2 and belongs to the adenylate kinase family. It plays a unique role in energy metabolism and energy transfer by regulating the ATP/ADP rate between the cytoplasma matrix and the mitochondria and it is increased expression during adipocyte and B cell differentiation(PMID:23020757). AK2 catalyzes the reversible transfer of a phosphoryl group from ATP to AMP(PMID:19043417). Defects in AK2 are the cause of reticular dysgenesis (RDYS)(PMID:19043416). It has 6 isoforms produced by alternative splicing.