验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells |
Positive IHC detected in | human colon cancer tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:500-1:2000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 1 publications below |
产品信息
12356-1-AP targets AKAP10 in WB, IHC, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB |
经测试反应性 | human |
文献引用反应性 | human |
免疫原 | AKAP10 fusion protein Ag3020 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | A kinase (PRKA) anchor protein 10 |
别名 | AKAP 10, AKAP10, D AKAP 2, D AKAP2, PRKA10 |
计算分子量 | 662 aa, 74 kDa |
观测分子量 | 74 kDa |
GenBank蛋白编号 | BC017055 |
基因名称 | AKAP10 |
Gene ID (NCBI) | 11216 |
RRID | AB_2225588 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | O43572 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for AKAP10 antibody 12356-1-AP | Download protocol |
IHC protocol for AKAP10 antibody 12356-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |