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验证数据展示

  • WB analysis of MCF-7 using 30552-1-AP

    WB analysis of MCF-7 using 30552-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 30552-1-AP (AKAP11-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 30552-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30552-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30552-1-AP (AKAP11-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AKAP11-Specific Polyclonal antibody

AKAP11-Specific Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30552-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

AKAP11, A-kinase anchor protein 11, AKAP220, AKAP-11, AKAP 220

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30552-1-AP targets AKAP11-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 AKAP11-Specific fusion protein Ag31539 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 A kinase (PRKA) anchor protein 11
别名 AKAP11, A-kinase anchor protein 11, AKAP220, AKAP-11, AKAP 220
计算分子量 211 kDa
观测分子量250 kDa
GenBank蛋白编号NM_016248
基因名称 AKAP11
Gene ID (NCBI) 11215
RRIDAB_3086360
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UKA4
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKAP11, also named as AKAP220, KIAA0629 and PRKA11, belongs to the AKAP110 family. AKAP11 binds to type II regulatory subunits of protein kinase A and anchors/targets them. The antibody is specific to AKAP11.

实验方案

Product Specific Protocols
WB protocol for AKAP11-Specific antibody 30552-1-APDownload protocol
IHC protocol for AKAP11-Specific antibody 30552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols