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AKAP11-Specific Polyclonal antibody

AKAP11-Specific Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 30552-1-AP

Print datasheet

Synonyms

AKAP11, A-kinase anchor protein 11, AKAP220, AKAP-11, AKAP 220

验证数据展示

  • WB analysis of MCF-7 using 30552-1-AP

    WB analysis of MCF-7 using 30552-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 30552-1-AP (AKAP11-Specific antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human intrahepatic cholangiocarcinoma using 30552-1-AP

    IHC staining of human intrahepatic cholangiocarcinoma using 30552-1-AP

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 30552-1-AP (AKAP11-Specific antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman intrahepatic cholangiocarcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30552-1-AP targets AKAP11-Specific in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen AKAP11-Specific fusion protein Ag31539 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name A kinase (PRKA) anchor protein 11
Synonyms AKAP11, A-kinase anchor protein 11, AKAP220, AKAP-11, AKAP 220
Calculated Molecular Weight 211 kDa
Observed Molecular Weight250 kDa
GenBank Accession NumberNM_016248
Gene Symbol AKAP11
Gene ID (NCBI) 11215
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UKA4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKAP11, also named as AKAP220, KIAA0629 and PRKA11, belongs to the AKAP110 family. AKAP11 binds to type II regulatory subunits of protein kinase A and anchors/targets them. The antibody is specific to AKAP11.

实验方案

Product Specific Protocols
WB protocol for AKAP11-Specific antibody 30552-1-APDownload protocol
IHC protocol for AKAP11-Specific antibody 30552-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols