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验证数据展示

  • WB analysis using 25199-1-AP (same clone as 25199-1-PBS)

    WB analysis using 25199-1-AP (same clone as 25199-1-PBS)

    rat brain tissue were subjected to SDS PAGE followed by western blot with 25199-1-AP (AKAP12 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 25199-1-AP (same clone as 25199-1-PBS)

    IHC staining of mouse brain using 25199-1-AP (same clone as 25199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKAP12 antibody included
  • IHC staining of mouse brain using 25199-1-AP (same clone as 25199-1-PBS)

    IHC staining of mouse brain using 25199-1-AP (same clone as 25199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKAP12 antibody included
  • IHC staining of human colon using 25199-1-AP (same clone as 25199-1-PBS)

    IHC staining of human colon using 25199-1-AP (same clone as 25199-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25199-1-AP (AKAP12 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25199-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with AKAP12 antibody included
  • Featured Product
  • KD/KO验证

AKAP12 Polyclonal antibody, PBS Only

AKAP12 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 25199-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy AKAP12 Ready-To-Use IHC Kit. AKAP12 primary antibody included.

产品信息

25199-1-PBS targets AKAP12 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 AKAP12 fusion protein Ag17769 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 A kinase (PRKA) anchor protein 12
别名
计算分子量 1782 aa, 191 kDa
观测分子量 200-300 kDa
GenBank蛋白编号BC157067
基因名称 AKAP12
Gene ID (NCBI) 9590
RRIDAB_2879954
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ02952
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AKAP12, also named as AKAP250, is a 1782 amino acid protein, which contains three AKAP domains. AKAP12 localizes in the cytoplasm and is expressed in endothelial cells, cultured fibroblasts and osteosarcoma, but not in platelets, leukocytes, monocytic cell lines or peripherical blood cells. AKAP12 as an anchoring protein that mediates the subcellular compartmentation of protein kinase A (PKA) and protein kinase C (PKC). AKAP12 expression is known to be suppressed in several human malignancies including breast, prostate, gastric and colon cancers. AKAP12 has three isoforms, namely AKAP12A, AKAP12B and AKAP12C, with molecular weights of 305 kDa, 287 kDa and 250 kDa respectively. (PMID: 30385176, PMID: 15258566)