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AKAP14 Polyclonal antibody

AKAP14 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14622-1-AP

Print datasheet

Synonyms

A kinase anchor protein 14, A kinase anchor protein 28 kDa, AKAP 14, AKAP 28, AKAP14, AKAP28

验证数据展示

  • WB analysis of Jurkat using 14622-1-AP

    WB analysis of Jurkat using 14622-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 14622-1-AP (AKAP14 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human kidney using 14622-1-AP

    IHC staining of human kidney using 14622-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 10x lens).

  • IHC staining of human kidney using 14622-1-AP

    IHC staining of human kidney using 14622-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 14622-1-AP (AKAP14 antibody) at dilution of 1:50 (under 40x lens).

经过测试的应用

Positive WB detected inJurkat cells, human testis tissue
Positive IHC detected inhuman kidney tissue, human brain tissue, human lung tissue, human ovary tissue, human placenta tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14622-1-AP targets AKAP14 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen AKAP14 fusion protein Ag6255 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name A kinase (PRKA) anchor protein 14
Synonyms A kinase anchor protein 14, A kinase anchor protein 28 kDa, AKAP 14, AKAP 28, AKAP14, AKAP28
Calculated Molecular Weight 23 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC066357
Gene Symbol AKAP14
Gene ID (NCBI) 158798
RRIDAB_2225760
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86UN6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AKAP14 antibody 14622-1-APDownload protocol
IHC protocol for AKAP14 antibody 14622-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols