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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IHC staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    IHC staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66255-1-Ig (AKAP3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    IHC staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66255-1-Ig (AKAP3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    IF Staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (66255-1-Ig, Clone: 3C11H9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

  • WB analysis of human testis using 66255-1-Ig (same clone as 66255-1-PBS)

    WB analysis of human testis using 66255-1-Ig (same clone as 66255-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66255-1-Ig (AKAP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    IF Staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (66255-1-Ig, Clone: 3C11H9 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

AKAP3 Monoclonal antibody, PBS Only

AKAP3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 66255-1-PBS

产品说明书

CloneNo. 3C11H9

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse

应用

WB, IHC, IF-P, Indirect ELISA

别名

A kinase anchor protein 3, AKAP 110, AKAP 3, AKAP110, AKAP3, Cancer/testis antigen 82, CT82, Fibrousheathin 1, Fibrousheathin I, FSP95, PRKA3, SOB1, Sperm oocyte binding protein

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66255-1-PBS targets AKAP3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 AKAP3 fusion protein Ag4885 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 A kinase (PRKA) anchor protein 3
别名 A kinase anchor protein 3, AKAP 110, AKAP 3, AKAP110, AKAP3, Cancer/testis antigen 82, CT82, Fibrousheathin 1, Fibrousheathin I, FSP95, PRKA3, SOB1, Sperm oocyte binding protein
计算分子量 95 kDa
观测分子量 100 kDa
GenBank蛋白编号BC047535
基因名称 AKAP3
Gene ID (NCBI) 10566
RRIDAB_2881643
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDO75969
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.