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AKAP3 Monoclonal antibody, PBS Only

AKAP3 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

3C11H9

Cat No : 66255-1-PBS

Print datasheet

Synonyms

A kinase anchor protein 3, AKAP 110, AKAP 3, AKAP110, AKAP3, Cancer/testis antigen 82, CT82, Fibrousheathin 1, Fibrousheathin I, FSP95, PRKA3, SOB1, Sperm oocyte binding protein

验证数据展示

  • IHC staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    IHC staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66255-1-Ig (AKAP3 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    IHC staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66255-1-Ig (AKAP3 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    IF Staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (66255-1-Ig, Clone: 3C11H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

  • WB analysis of human testis using 66255-1-Ig (same clone as 66255-1-PBS)

    WB analysis of human testis using 66255-1-Ig (same clone as 66255-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 66255-1-Ig (AKAP3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

  • IF Staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    IF Staining of mouse testis using 66255-1-Ig (same clone as 66255-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using AKAP3 antibody (66255-1-Ig, Clone: 3C11H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66255-1-PBS in a different storage buffer formulation.

产品信息

66255-1-PBS targets AKAP3 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human, mouse
Immunogen AKAP3 fusion protein Ag4885 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name A kinase (PRKA) anchor protein 3
Synonyms A kinase anchor protein 3, AKAP 110, AKAP 3, AKAP110, AKAP3, Cancer/testis antigen 82, CT82, Fibrousheathin 1, Fibrousheathin I, FSP95, PRKA3, SOB1, Sperm oocyte binding protein
Calculated Molecular Weight 95 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC047535
Gene Symbol AKAP3
Gene ID (NCBI) 10566
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDO75969
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

AKAP3 is a member of A-kinase anchoring proteins (AKAPs), a family of functionally related proteins that target protein kinase A to discrete locations within the cell. AKAP3 is reported to participate in protein-protein interactions with the R-subunit of the protein kinase A as well as sperm-associated proteins. AKAP3 is expressed in spermatozoa and localized to the acrosomal region of the sperm head as well as the length of the principal piece. It may function as a regulator of motility, capacitation, and the acrosome reaction.