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验证数据展示

  • WB analysis using 31542-1-AP

    WB analysis using 31542-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31542-1-AP (AKAP8 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

AKAP8 Polyclonal antibody

AKAP8 Polyclonal Antibody for WB, ELISA
Cat No. 31542-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human

应用

WB, ELISA

别名

A kinase anchor protein 8, A kinase anchor protein 95 kDa, AKAP 8, AKAP 95, AKAP8, AKAP95, DKFZp586B1222

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HL-60 cells, HeLa cells, HepG2 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31542-1-AP targets AKAP8 in WB, ELISA applications and shows reactivity with Human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human
免疫原 AKAP8 fusion protein Ag35816 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 A kinase (PRKA) anchor protein 8
别名 A kinase anchor protein 8, A kinase anchor protein 95 kDa, AKAP 8, AKAP 95, AKAP8, AKAP95, DKFZp586B1222
计算分子量76kDa，692aa
观测分子量 95 kDa
GenBank蛋白编号NM_005858.3
基因名称 AKAP8
Gene ID (NCBI) 10270
RRIDAB_3670031
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDO43823
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKAP8 (A-kinase anchor protein 8), also called AKAP95, is a A-kinase anchor protein that directs activity of protein kinase A by tethering the enzyme near its physiologic substrates (PMID: 9473338; 10601332). It is associated with chromatin and nuclear matrix which has a remarkable activity in promoting chromatin transcription (PMID: 31980632), and it is also required for cell cycle G2/M transition and histone deacetylation during mitosis (PMID: 16980585).

实验方案

Product Specific Protocols
WB protocol for AKAP8 antibody 31542-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols