• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
  • Featured Product
  • KD/KO Validated

AKAP8L Polyclonal antibody

AKAP8L Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 10103-1-AP

Print datasheet

Synonyms

HA95, A-kinase anchor protein 8-like, AKAP95, AKAP8-like protein, AKAP8 like protein

验证数据展示

  • WB analysis of PC-3 using 10103-1-AP

    WB analysis of PC-3 using 10103-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10103-1-AP (AKAP8L Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 10103-1-AP

    IF Staining of HEK-293 using 10103-1-AP

    Immunofluorescent analysis of HEK-293 cells using 10103-1-AP (AKAP8L antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inPC-3 cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10103-1-AP targets AKAP8L in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen AKAP8L fusion protein Ag0145 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name A kinase (PRKA) anchor protein 8-like
Synonyms HA95, A-kinase anchor protein 8-like, AKAP95, AKAP8-like protein, AKAP8 like protein
Calculated Molecular Weight 72 kDa
Observed Molecular Weight 72 kDa
GenBank Accession NumberBC000713
Gene Symbol AKAP8L
Gene ID (NCBI) 26993
RRIDAB_2258197
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULX6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKAP8L, also named as NAKAP, or NAKAP95, is a 646 amino acid protein, which contains two C2H2 AKAP95-type zinc fingers and belongs to the AKAP95 family. AKAP8L localizes in the nucleus matrix and is expressed in the brain cortex. AKAP8L could play a role in constitutive transport element (CTE)-mediated gene expression. AKAP8L may be involved in nuclear envelope breakdown and chromatin condensation. It also may regulate the initiation phase of DNA replication when associated with TMPO-beta.

实验方案

Product Specific Protocols
WB protocol for AKAP8L antibody 10103-1-APDownload protocol
IF protocol for AKAP8L antibody 10103-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Retrovirology

The role of A-kinase anchoring protein 95-like protein in annealing of tRNALys3 to HIV-1 RNA.

Authors - Li Xing
  • KD Validated
View Article
humanWB

RNA Biol

hnRNPH1-MTR4 complex-mediated regulation of NEAT1v2 stability is critical for IL8 expression.

Authors - Tanzina Tanu
  • KD Validated
View Article