验证数据展示
经过测试的应用
| Positive WB detected in | PC-3 cells |
| Positive IF/ICC detected in | HEK-293 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 2 publications below |
产品信息
10103-1-AP targets AKAP8L in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human |
| 文献引用反应性 | human |
| 免疫原 | AKAP8L fusion protein Ag0145 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | A kinase (PRKA) anchor protein 8-like |
| 别名 | HA95, A-kinase anchor protein 8-like, AKAP95, AKAP8-like protein, AKAP8 like protein |
| 计算分子量 | 72 kDa |
| 观测分子量 | 72 kDa |
| GenBank蛋白编号 | BC000713 |
| 基因名称 | AKAP8L |
| Gene ID (NCBI) | 26993 |
| RRID | AB_2258197 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9ULX6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AKAP8L, also named as NAKAP, or NAKAP95, is a 646 amino acid protein, which contains two C2H2 AKAP95-type zinc fingers and belongs to the AKAP95 family. AKAP8L localizes in the nucleus matrix and is expressed in the brain cortex. AKAP8L could play a role in constitutive transport element (CTE)-mediated gene expression. AKAP8L may be involved in nuclear envelope breakdown and chromatin condensation. It also may regulate the initiation phase of DNA replication when associated with TMPO-beta.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AKAP8L antibody 10103-1-AP | Download protocol |
| IF protocol for AKAP8L antibody 10103-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Retrovirology The role of A-kinase anchoring protein 95-like protein in annealing of tRNALys3 to HIV-1 RNA.
| ||
RNA Biol hnRNPH1-MTR4 complex-mediated regulation of NEAT1v2 stability is critical for IL8 expression.
|