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AKD2 Polyclonal antibody

AKD2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27554-1-AP

Print datasheet

Synonyms

Adenylate kinase 9, AK 9, AK9, AKD1, AKD2, C6orf199, C6orf224, RP1 70A9.1

验证数据展示

  • WB analysis using 27554-1-AP

    WB analysis using 27554-1-AP

    Various lysates lysate were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of SMMC-7721 using 27554-1-AP

    WB analysis of SMMC-7721 using 27554-1-AP

    SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 27554-1-AP (AKD2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human heart using 27554-1-AP

    IHC staining of human heart using 27554-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human heart using 27554-1-AP

    IHC staining of human heart using 27554-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27554-1-AP (AKD2 antibody) at dilution of 1:200 (under 40x lens).

经过测试的应用

Positive WB detected inSMMC-7721 cells, HepG2 cells, HuH-7 cells
Positive IHC detected inhuman heart tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

27554-1-AP targets AKD2 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity Human
Cited Reactivityhuman
Immunogen AKD2 fusion protein Ag25805 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name adenylate kinase domain containing 2
Synonyms Adenylate kinase 9, AK 9, AK9, AKD1, AKD2, C6orf199, C6orf224, RP1 70A9.1
Calculated Molecular Weight221 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberBC022031
Gene Symbol AKD2
Gene ID (NCBI) 221264
RRIDAB_2880904
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ5TCS8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AK9, also named as adenylate kinase 9, belongs to adenylate kinase family, which is a small, ususlly monomeric, enzyme found in every living thing due to its crucial role in energenic metabolism(PMID: 30666486). AK9 is a nucleoside mono- and diphosphate kinase due to its broad phosphatase activity, the local concentration of substrates probably influences catalytic activity(PMID: 24495878). This antibody can detect 45-50 kDa band of AK9(PMID: 23416111).

实验方案

Product Specific Protocols
WB protocol for AKD2 antibody 27554-1-APDownload protocol
IHC protocol for AKD2 antibody 27554-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Mol Carcinog

Integrative analyses of N6-methyladenosine-associated single-nucleotide polymorphisms (m6A-SNPs) identify tumor suppressor gene AK9 in lung cancer

Authors - Tingting Hua
View Article