验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells, 4T1 cells |
Planning an IHC experiment? We recommend our IHCeasy AKR1B1 Ready-To-Use IHC Kit. AKR1B1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 2 publications below |
产品信息
67498-1-Ig targets AKR1B1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human, Mouse |
| 文献引用反应性 | human, mouse |
| 免疫原 | AKR1B1 fusion protein Ag7571 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | aldo-keto reductase family 1, member B1 (aldose reductase) |
| 别名 | ADR, AKR1B1, Aldehyde reductase, Aldose reductase, ALDR1, ALR2, AR |
| 计算分子量 | 36 kDa |
| 观测分子量 | 34 kDa |
| GenBank蛋白编号 | BC000260 |
| 基因名称 | AKR1B1 |
| Gene ID (NCBI) | 231 |
| RRID | AB_2882722 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P15121 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AKR1B1 antibody 67498-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol An Advanced Systems Pharmacology Strategy Reveals AKR1B1, MMP2, PTGER3 as Key Genes in the Competing Endogenous RNA Network of Compound Kushen Injection Treating Gastric Carcinoma by Integrated Bioinformatics and Experimental Verification | ||
Pharmacol Res Inulin-type fructans change the gut microbiota and prevent the development of diabetic nephropathy. |
