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AKR1C4 Polyclonal antibody

AKR1C4 Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC, WB, ELISA and More (1)

Conjugate

Unconjugated

Cat No : 18852-1-AP

Print datasheet

Synonyms

3 alpha HSD, 3 alpha HSD1, AKR1C4, C11, CDR, CHDR, Chlordecone reductase, DD 4, DD4, Dihydrodiol dehydrogenase 4, HAKRA

验证数据展示

  • WB analysis using 18852-1-AP

    WB analysis using 18852-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18852-1-AP (AKR1C4 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 18852-1-AP

    IF Staining of HepG2 using 18852-1-AP

    Immunofluorescent analysis of HepG2 cells, using AKR1C4 antibody 18852-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

经过测试的应用

Positive WB detected inHeLa cells, mouse liver tissue, HepG2 cells
Positive IF detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 18852-1-AP is AKR1C4 Fusion Protein expressed in E. coli.

Tested Applications IF/ICC, WB, ELISA Application Description
Cited ApplicationsWB, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen AKR1C4 fusion protein Ag13444 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aldo-keto reductase family 1, member C4 (chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4)
Synonyms 3 alpha HSD, 3 alpha HSD1, AKR1C4, C11, CDR, CHDR, Chlordecone reductase, DD 4, DD4, Dihydrodiol dehydrogenase 4, HAKRA
Calculated Molecular Weight 323 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC020744
Gene Symbol AKR1C4
Gene ID (NCBI) 1109
RRIDAB_10665359
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP17516
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AKR1C4 antibody 18852-1-APDownload protocol
IF protocol for AKR1C4 antibody 18852-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IHC

Redox Biol

Ubiquitination as a key regulatory mechanism for O3-induced cutaneous redox inflammasome activation

Authors - Francesca Ferrara
View Article