验证数据展示
经过测试的应用
| Positive WB detected in | human liver tissue, HepG2 cells, human brain tissue, NIH/3T3 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
产品信息
13209-1-AP targets AKR7A3 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | rat |
| 免疫原 | AKR7A3 fusion protein Ag3991 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | aldo-keto reductase family 7, member A3 (aflatoxin aldehyde reductase) |
| 别名 | EC:1.-.-.-, AFB1-AR 2, AFB1 AR 2, AFB1 aldehyde reductase 2, AFAR2 |
| 计算分子量 | 331 aa, 37 kDa |
| 观测分子量 | 37 kDa and 55-60 kDa |
| GenBank蛋白编号 | BC025709 |
| 基因名称 | AKR7A3 |
| Gene ID (NCBI) | 22977 |
| RRID | AB_2224549 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95154 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AKR7A3 belongs to the aldo‐keto reductase (AKR) superfamily, whose primary role is to reduce aldehydes and ketones to generate primary and secondary alcohols, respectively. These enzymes have been shown to play crucial roles in drug metabolism, carcinogen metabolism, and cellular metabolism. Growing evidence suggests that AKR7A3 can play an essential role in the occurrence of cancers, including breast and liver cancers. (PMID: 36951402)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AKR7A3 antibody 13209-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
