AKT1-Specific Recombinant antibody

AKT1-Specific Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 80457-1-RR

产品说明书

CloneNo. 4I5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ELISA

AKT1 (C-terminal), AKT, AKT (pan), AKT 1, AKT pan

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHEK-293 cells, HEK-293T cells, NIH/3T3 cells, Calyculin A treated HepG2 cells, Calyculin A treated HEK-293 cells, HeLa cells, Calyculin A treated HeLa cells, Calyculin A treated NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy AKT Ready-To-Use IHC Kit. AKT primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 4 publications below

产品信息

80457-1-RR targets AKT1-Specific in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human, mouse
文献引用反应性human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 v-akt murine thymoma viral oncogene homolog 1
别名 AKT1 (C-terminal), AKT, AKT (pan), AKT 1, AKT pan
观测分子量56-62 kDa
GenBank蛋白编号NM_005163
基因名称 AKT1
Gene ID (NCBI) 207
RRIDAB_2918893
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP31749
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AKT is a serine/threonine kinase and it participates in the key role of the PI3K signaling pathway. Phosphatidylinositol-3 kinase (PI3K) is the key regulator of AKT activation. The recruitment of inactive AKT protein to PIP3-rich areas of the plasma membrane results in a conformational change that exposes the activation loop of AKT. AKT's activating kinase, phosphoinositide-dependent protein kinase (PDK1) , is also recruited to PIP3 microdomains. PDK1 phosphorylates AKT on threonine 308 (Thr308) of the exposed activation loop, activating AKT and leading to a second phosphorylation of AKT at serine 473 (Ser473) by a kinase presumed to be mTORC2 that further potentiates kinase activity. Active AKT will phosphorylate various downstream protein targets that control cell growth and translational control and act to suppress apoptosis. (PMID: 31594388, PMID: 30808672). 80457-1-RR specifically recognizes AKT1.

实验方案

Product Specific Protocols
WB protocol for AKT1-Specific antibody 80457-1-RRDownload protocol
IHC protocol for AKT1-Specific antibody 80457-1-RRDownload protocol
IF protocol for AKT1-Specific antibody 80457-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Redox Biol

CircSV2b participates in oxidative stress regulation through miR-5107-5p-Foxk1-Akt1 axis in Parkinson's disease

Authors - Quancheng Cheng
mouseWB

Biol Pharm Bull

Dictamnine ameliorates DNFB-induced atopic dermatitis like skin lesions in mice by inhibiting M1 macrophage polarization and promoting autophagy

Authors - Yihan Huang
humanWB

Nat Commun

Multi-protomics analysis identified host cellular pathways perturbed by tick-borne encephalitis virus infection

Authors - Liyan Sui
humanWB

Oncogene

The protection of UCK2 protein stability by GART maintains pyrimidine salvage synthesis for HCC growth under glucose limitation

Authors - Nannan Sha
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