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AKTIP Polyclonal antibody

AKTIP Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 14860-1-AP

Print datasheet

Synonyms

FTS, Ft1, AKT-interacting protein

验证数据展示

  • WB analysis of HepG2 using 14860-1-AP

    WB analysis of HepG2 using 14860-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 14860-1-AP (AKTIP antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IP experiment of HepG2 using 14860-1-AP

    IP experiment of HepG2 using 14860-1-AP

    IP result of anti-AKTIP (IP:14860-1-AP, 4ug; Detection:14860-1-AP 1:300) with HepG2 cells lysate 1680 ug.

  • IF Staining of SH-SY5Y using 14860-1-AP

    IF Staining of SH-SY5Y using 14860-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed SH-SY5Y cells using 14860-1-AP (AKTIP antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHepG2 cells
Positive IP detected inHepG2 cells
Positive IF/ICC detected inSH-SY5Y cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14860-1-AP targets AKTIP in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen AKTIP fusion protein Ag6662 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name AKT interacting protein
Synonyms FTS, Ft1, AKT-interacting protein
Calculated Molecular Weight 32 kDa
Observed Molecular Weight 33 kDa
GenBank Accession NumberBC001134
Gene Symbol AKTIP
Gene ID (NCBI) 64400
RRIDAB_2878087
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H8T0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AKTIP antibody 14860-1-APDownload protocol
IF protocol for AKTIP antibody 14860-1-APDownload protocol
IP protocol for AKTIP antibody 14860-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cell Rep

AKTIP loss is enriched in ERα-positive breast cancer for tumorigenesis and confers endocrine resistance

Authors - Angel S N Ng
  • KD Validated
View Article