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验证数据展示

  • WB analysis using 30539-1-AP

    WB analysis using 30539-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30539-1-AP (ALAS2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 30539-1-AP

    IF Staining of HEK-293 using 30539-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using ALAS2 antibody (30539-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

ALAS2 Polyclonal antibody

ALAS2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 30539-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

ALAS-E, ALASE, ALAS E, ALAS 2, 5-aminolevulinic acid synthase 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inK-562 cells, NIH/3T3 cells, mouse brain tissue, mouse heart tissue
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30539-1-AP targets ALAS2 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 ALAS2 fusion protein Ag30308 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 aminolevulinate, delta-, synthase 2
别名 ALAS-E, ALASE, ALAS E, ALAS 2, 5-aminolevulinic acid synthase 2
计算分子量 587 aa, 65 kDa
观测分子量65 kDa
GenBank蛋白编号BC030230
基因名称 ALAS2
Gene ID (NCBI) 212
RRIDAB_3086355
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP22557
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The heme biosynthetic pathway begins from delta aminolevulinic acid synthase (ALAS) catalyzing the condensation of glycine and succinyl-CoA to delta aminolevulinic acid (ALA) in the mitochondria. ALAS is coded by two genes: ALAS1 and ALAS2 . ALAS1 is ubiquitously expressed in all cells, and the negative feedback is regulated by the heme pool; however, ALAS2 is specifically expressed only in erythroid cells.

实验方案

Product Specific Protocols
WB protocol for ALAS2 antibody 30539-1-APDownload protocol
IF protocol for ALAS2 antibody 30539-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols