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Mouse ALCAM Recombinant antibody, PBS Only (Capture)

ALCAM Uni-rAbTM Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241945A4

Cat no : 84528-1-PBS

Print datasheet

Synonyms

Protein DM-GRASP, CD166 antigen, CD166, BEN, Activated leukocyte cell adhesion molecule

验证数据展示

  • WB analysis of mouse lung using 84528-1-RR (same clone as 84528-1-PBS)

    WB analysis of mouse lung using 84528-1-RR (same clone as 84528-1-PBS)

    mouse lung tissue was subjected to SDS PAGE followed by western blot with 84528-1-RR (ALCAM antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 84528-1-RR (same clone as 84528-1-PBS)

    IHC staining of mouse liver using 84528-1-RR (same clone as 84528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84528-1-RR (ALCAM antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse liver using 84528-1-RR (same clone as 84528-1-PBS)

    IHC staining of mouse liver using 84528-1-RR (same clone as 84528-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84528-1-RR (ALCAM antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84528-1-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP01379-1

    Cytometric bead array standard curve of MP01379-1

    Cytometric bead array standard curve of MP01379-1, MOUSE ALCAM Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84528-1-PBS. Detection antibody: 84528-3-PBS. Standard: Eg1524. Range: 0.781-100 ng/mL.

产品信息

84528-1-PBS targets ALCAM as part of a matched antibody pair:

MP01379-1: 84528-1-PBS capture and 84528-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity mouse
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name activated leukocyte cell adhesion molecule
Synonyms Protein DM-GRASP, CD166 antigen, CD166, BEN, Activated leukocyte cell adhesion molecule
Calculated Molecular Weight65kDa
Observed Molecular Weight90-100 kDa
GenBank Accession NumberNM_009655.3
Gene Symbol Alcam
Gene ID (NCBI) 11658
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ61490
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Activated leukocyte cell adhesion molecule (ALCAM, also known as CD166) is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is involved in cell-cell adhesion through homophilic and heterophilic (to CD6) interactions. ALCAM is widely expressed in a variety of normal tissues. Altered ALCAM expression has been associated with the differentiation state and progression in some neoplasms including melanoma, prostate, colorectal, and breast cancers (PMID: 20461761; 18172759).