ALDH1A1 Recombinant antibody

ALDH1A1 Recombinant Antibody for WB, IHC, ELISA
Cat No. 83830-2-RR

产品说明书

CloneNo. 240995F4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

ALDH1 A1, ALDH 1A1, Aldehyde dehydrogenase 1A1, ALDC, 3-deoxyglucosone dehydrogenase

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inK-562 cells, mouse liver tissue, mouse lung tissue
Positive IHC detected inhuman cervical squamous cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ALDH1A1 Ready-To-Use IHC Kit. ALDH1A1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83830-2-RR targets ALDH1A1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 ALDH1A1 fusion protein Ag8665 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 aldehyde dehydrogenase 1 family, member A1
别名 ALDH1 A1, ALDH 1A1, Aldehyde dehydrogenase 1A1, ALDC, 3-deoxyglucosone dehydrogenase
计算分子量 501 aa, 55 kDa
观测分子量 50-55 kDa
GenBank蛋白编号BC001505
基因名称 ALDH1A1
Gene ID (NCBI) 216
RRIDAB_3671414
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP00352
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALDH1A1(Aldehyde dehydrogenase family 1 member A1 ), also named as ALDC, ALDH1 and PUMB1, belongs to the aldehyde dehydrogenase family. The ALDH1A1 gene encodes a liver cytosolic isoform of acetaldehyde dehydrogenase, an enzyme involved in the major pathway of alcohol metabolism after alcohol dehydrogenase. ALDH1A1 plays a critical role in protection against oxidative stress-induced cytotoxicity in lens epithelial cells(PMID:19296407). And it is important for multiple biological activities including drug resistance, cell differentiation, and oxidative stress response(PMID:19025616). As a novel cancer stem cell marker, ALDH1A1 can be used for tumors whose corresponding normal tissues express ALDH1 in relatively restricted or limited levels such as breast, lung, ovarian or colon cancer(PMID: 20422001).

实验方案

Product Specific Protocols
WB protocol for ALDH1A1 antibody 83830-2-RRDownload protocol
IHC protocol for ALDH1A1 antibody 83830-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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