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ALDH1L1 Monoclonal antibody

ALDH1L1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse, pig

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1C1A3

Cat no : 68018-1-Ig

Print datasheet

Synonyms

10 FTHFDH, ALDH1L1, DKFZp781N0997, FTHFD

验证数据展示

  • WB analysis of human colostrum using 68018-1-Ig

    WB analysis of human colostrum using 68018-1-Ig

    human colostrum were subjected to SDS PAGE followed by western blot with 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 68018-1-Ig

    IHC staining of mouse brain using 68018-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 68018-1-Ig

    IHC staining of mouse brain using 68018-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 68018-1-Ig (ALDH1L1 antibody) at dilution of 1:32000 (under 4x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

经过测试的应用

Positive WB detected inhuman colostrum, pig liver tissue, rat liver tissue, mouse liver tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:16000-1:64000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68018-1-Ig targets ALDH1L1 in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse, pig samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity Human, rat, mouse, pig
Immunogen ALDH1L1 fusion protein Ag13190 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name aldehyde dehydrogenase 1 family, member L1
Synonyms 10 FTHFDH, ALDH1L1, DKFZp781N0997, FTHFD
Calculated Molecular Weight 99 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC027241
Gene Symbol ALDH1L1
Gene ID (NCBI) 10840
RRIDAB_2918764
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO75891
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aldehyde dehydrogenase 1 family member L1 (ALDH1L1), belongs to the aldehyde dehydrogenase family of proteins and is responsible for formate oxidation in vivo. Loss of ALDH1L1 is associated with decreased apoptosis, increased cell motility, and cancer progression. ALDH1L1 catalyzes the conversion of 10-formyltetrahydrofolate, nicotinamide adenine dinucleotide phosphate (NADP+), and water to tetrahydrofolate, NADPH, and carbon dioxide. ALDH1L1 has some isoforms with MW of 99, 88 and 55 kDa.

实验方案

Product Specific Protocols
WB protocol for ALDH1L1 antibody 68018-1-IgDownload protocol
IHC protocol for ALDH1L1 antibody 68018-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols