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ALDH8A1 Polyclonal antibody

ALDH8A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 21602-1-AP

Print datasheet

Synonyms

EC:1.2.1.32, ALDH8A 1, ALDH12, 2-aminomuconic semialdehyde dehydrogenase

验证数据展示

  • WB analysis using 21602-1-AP

    WB analysis using 21602-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 21602-1-AP (ALDH8A1 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • IHC staining of human hepatocirrhosis using 21602-1-AP

    IHC staining of human hepatocirrhosis using 21602-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human hepatocirrhosis using 21602-1-AP

    IHC staining of human hepatocirrhosis using 21602-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HepG2 using 21602-1-AP

    IF Staining of HepG2 using 21602-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 21602-1-AP (ALDH8A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inL02 cells, mouse kidney tissue, mouse liver tissue, rat liver tissue
Positive IHC detected inhuman hepatocirrhosis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:5000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21602-1-AP targets ALDH8A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ALDH8A1 fusion protein Ag16214 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aldehyde dehydrogenase 8 family, member A1
Synonyms EC:1.2.1.32, ALDH8A 1, ALDH12, 2-aminomuconic semialdehyde dehydrogenase
Calculated Molecular Weight 487 aa, 53 kDa
Observed Molecular Weight 53 kDa
GenBank Accession NumberBC113862
Gene Symbol ALDH8A1
Gene ID (NCBI) 64577
RRIDAB_10733887
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H2A2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ALDH8A1 antibody 21602-1-APDownload protocol
IHC protocol for ALDH8A1 antibody 21602-1-APDownload protocol
IF protocol for ALDH8A1 antibody 21602-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols