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ALDH9A1 Polyclonal antibody

ALDH9A1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26621-1-AP

Print datasheet

Synonyms

EC:1.2.1.19, ALDH9, ALDH7, ALDH4, 4-trimethylaminobutyraldehyde dehydrogenase, N-terminally processed

验证数据展示

  • WB analysis of NIH/3T3 using 26621-1-AP

    WB analysis of NIH/3T3 using 26621-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 26621-1-AP

    IHC staining of human liver cancer using 26621-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALDH9A1 antibody included
  • IHC staining of human liver cancer using 26621-1-AP

    IHC staining of human liver cancer using 26621-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26621-1-AP (ALDH9A1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALDH9A1 antibody included
  • IF Staining of HeLa using 26621-1-AP

    IF Staining of HeLa using 26621-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using ALDH9A1 antibody (26621-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inNIH/3T3 cells, HEK-293 cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ALDH9A1 Ready-To-Use IHC Kit. ALDH9A1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:100-1:400
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 2 publications below

产品信息

26621-1-AP targets ALDH9A1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen ALDH9A1 fusion protein Ag24312 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name aldehyde dehydrogenase 9 family, member A1
Synonyms EC:1.2.1.19, ALDH9, ALDH7, ALDH4, 4-trimethylaminobutyraldehyde dehydrogenase, N-terminally processed
Calculated Molecular Weight 518 aa, 56 kDa
Observed Molecular Weight 46 kDa
GenBank Accession NumberBC151140
Gene Symbol ALDH9A1
Gene ID (NCBI) 223
RRIDAB_2880577
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP49189
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Aldehyde dehydrogenase 9 family member A1(ALDH9A1), belongs to the aldehyde dehydrogenase family of proteins. ALDH9A1 has a high activity for oxidation of gamma-aminobutyraldehyde and other amino aldehydes. ALDH9A1 catalyzes the dehydrogenation of gamma-aminobutyraldehyde to gamma-aminobutyric acid. ALDH9A1 has 3 isoforms with the molecular mass of 46-56 kDa.

实验方案

Product Specific Protocols
WB protocol for ALDH9A1 antibody 26621-1-APDownload protocol
IHC protocol for ALDH9A1 antibody 26621-1-APDownload protocol
IF protocol for ALDH9A1 antibody 26621-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancers (Basel)

Mitochondrial Protein Cox7b Is a Metabolic Sensor Driving Brain-Specific Metastasis of Human Breast Cancer Cells

Authors - Marine C N M Blackman
View Article
humanWB

Proteomes

Utilizing Optimized Tools to Investigate PTM Crosstalk: Identifying Potential PTM Crosstalk of Acetylated Mitochondrial Proteins.

Authors - Henrick Horita
View Article