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  • Featured Product
  • KD/KO Validated

ALG1 Polyclonal antibody

ALG1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 12872-1-AP

Print datasheet

Synonyms

GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase, EC:2.4.1.142, Chitobiosyldiphosphodolichol beta-mannosyltransferase, Beta-1,4-mannosyltransferase, Beta 1,4 mannosyltransferase

验证数据展示

  • WB analysis of HeLa using 12872-1-AP

    WB analysis of HeLa using 12872-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 12872-1-AP (ALG1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IP experiment of HeLa using 12872-1-AP

    IP experiment of HeLa using 12872-1-AP

    IP result of anti-ALG1 (IP:12872-1-AP, 4ug; Detection:12872-1-AP 1:500) with HeLa cells lysate 3080 ug.

  • IHC staining of human gliomas using 12872-1-AP

    IHC staining of human gliomas using 12872-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human gliomas using 12872-1-AP

    IHC staining of human gliomas using 12872-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 12872-1-AP (ALG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 12872-1-AP

    IF Staining of HeLa using 12872-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12872-1-AP (ALG1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12872-1-AP targets ALG1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, IP, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Immunogen ALG1 fusion protein Ag3540 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name asparagine-linked glycosylation 1, beta-1,4-mannosyltransferase homolog (S. cerevisiae)
Synonyms GDP-Man:GlcNAc2-PP-dolichol mannosyltransferase, EC:2.4.1.142, Chitobiosyldiphosphodolichol beta-mannosyltransferase, Beta-1,4-mannosyltransferase, Beta 1,4 mannosyltransferase
Calculated Molecular Weight 464 aa, 53 kDa
Observed Molecular Weight 47-53 kDa
GenBank Accession NumberBC031095
Gene Symbol ALG1
Gene ID (NCBI) 56052
RRIDAB_2289541
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BT22
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ALG1 antibody 12872-1-APDownload protocol
IHC protocol for ALG1 antibody 12872-1-APDownload protocol
IF protocol for ALG1 antibody 12872-1-APDownload protocol
IP protocol for ALG1 antibody 12872-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS One

O-GlcNAc-specific antibody CTD110.6 cross-reacts with N-GlcNAc2-modified proteins induced under glucose deprivation.

Authors - Isono Takahiro T
  • KD Validated
View Article
humanWB

J Agric Food Chem

Structural Characterization of Zinc-Sucrose Complex and Its Ability to Promote Zinc Absorption in Caco-2 Monolayer Cells and Mice

Authors - Nan Li
View Article
humanWB

Int J Mol Sci

Targeted Proteomics Reveals Quantitative Differences in Low-Abundance Glycosyltransferases of Patients with Congenital Disorders of Glycosylation

Authors - Roman Sakson
View Article