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  • Featured Product
  • KD/KO Validated

ALG13 Polyclonal antibody

ALG13 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 20810-1-AP

Print datasheet

Synonyms

ALG13, CXorf45, GLT28D1, MDS031, YGL047W

验证数据展示

  • WB analysis of HepG2 using 20810-1-AP

    WB analysis of HepG2 using 20810-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 20810-1-AP (ALG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach using 20810-1-AP

    IHC staining of human stomach using 20810-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 20810-1-AP (ALG13 antibody) at dilution of 1:100 (under 10x lens).

  • IHC staining of human stomach using 20810-1-AP

    IHC staining of human stomach using 20810-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 20810-1-AP (ALG13 antibody) at dilution of 1:100 (under 40x lens).

经过测试的应用

Positive WB detected inHepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 20810-1-AP is ALG13 Fusion Protein expressed in E. coli.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF, IHC
Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Immunogen ALG13 fusion protein Ag14763 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name asparagine-linked glycosylation 13 homolog
Synonyms ALG13, CXorf45, GLT28D1, MDS031, YGL047W
Calculated Molecular Weight 165 aa, 18 kDa
Observed Molecular Weight 130-135 kDa
GenBank Accession NumberBC005336
Gene Symbol ALG13
Gene ID (NCBI) 79868
RRIDAB_10694830
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP73
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ALG13 antibody 20810-1-APDownload protocol
IHC protocol for ALG13 antibody 20810-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

J Inherit Metab Dis

Predominant and novel de novo variants in 29 individuals with ALG13 deficiency: Clinical description, biomarker status, biochemical analysis, and treatment suggestions.

Authors - Bobby G Ng
View Article
mouseIF

Nephron

Dysregulation of the Expression of Asparagine-Linked Glycosylation 13 Short Isoform 2 Affects Nephrin Function by Altering Its N-Linked Glycosylation.

Authors - Teresa Esposito
View Article
mouseWB,IHC,IF

Neuroscience

ALG13 Deficiency Associated with Increased Seizure Susceptibility and Severity.

Authors - Peng Gao
  • KO Validated
View Article