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验证数据展示

  • IHC staining of mouse brain using 28738-1-AP

    IHC staining of mouse brain using 28738-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28738-1-AP (ALG13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse brain using 28738-1-AP

    IHC staining of mouse brain using 28738-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28738-1-AP (ALG13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of mouse brain using 28738-1-AP

    IF Staining of mouse brain using 28738-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using ALG13 antibody (28738-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • WB analysis using 28738-1-AP

    WB analysis using 28738-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 28738-1-AP (ALG13 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

ALG13 Polyclonal antibody

ALG13 Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 28738-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse and More (1)

应用

WB, IHC, IF-P, ELISA

别名

ALG13, CXorf45, FLJ23018, GLT28D1, MDS031, YGL047W

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inJurkat cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

28738-1-AP targets ALG13 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
文献引用应用WB
经测试反应性 Human, mouse
文献引用反应性human
免疫原 ALG13 fusion protein Ag30299 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 asparagine-linked glycosylation 13 homolog
别名 ALG13, CXorf45, FLJ23018, GLT28D1, MDS031, YGL047W
计算分子量 165 aa, 18 kDa
观测分子量130-135,18 kDa
GenBank蛋白编号BC005336
基因名称 ALG13
Gene ID (NCBI) 79868
RRIDAB_2918198
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NP73
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ALG13 antibody 28738-1-APDownload protocol
IHC protocol for ALG13 antibody 28738-1-APDownload protocol
IF protocol for ALG13 antibody 28738-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Front Cell Dev Biol

An in vitro assay for enzymatic studies on human ALG13/14 heterodimeric UDP-N-acetylglucosamine transferase

Authors - Chun-Di Wang
View Article