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ALG2 Polyclonal antibody

ALG2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15092-1-AP

Print datasheet

Synonyms

ALG2, CDGIi, hALPG2, NET38

验证数据展示

  • IHC staining of human pancreas cancer using 15092-1-AP

    IHC staining of human pancreas cancer using 15092-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15092-1-AP (ALG2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human pancreas cancer using 15092-1-AP

    IHC staining of human pancreas cancer using 15092-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 15092-1-AP (ALG2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 15092-1-AP

    WB analysis using 15092-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15092-1-AP (ALG2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, human placenta tissue
Positive IHC detected inhuman pancreas cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

15092-1-AP targets ALG2 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen ALG2 fusion protein Ag4833 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name asparagine-linked glycosylation 2, alpha-1,3-mannosyltransferase homolog (S. cerevisiae)
Synonyms ALG2, CDGIi, hALPG2, NET38
Calculated Molecular Weight 22 kDa
Observed Molecular Weight 47 kDa, 37 kDa
GenBank Accession NumberBC015126
Gene Symbol ALG2
Gene ID (NCBI) 85365
RRIDAB_2878108
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H553
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ALG2 antibody 15092-1-APDownload protocol
IHC protocol for ALG2 antibody 15092-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

bioRxiv

A mechanism that transduces lysosomal damage signals to stress granule formation for cell survival

Authors - Jacob Duran
View Article