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ALG3 Polyclonal antibody
ALG3 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HepG2 cells |
| Positive IHC detected in | human thyroid cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ALG3 Ready-To-Use IHC Kit. ALG3 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20290-1-AP targets ALG3 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | ALG3 fusion protein Ag14059 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | asparagine-linked glycosylation 3, alpha-1,3- mannosyltransferase homolog (S. cerevisiae) |
| Synonyms | ALG3, CDGS4, D16Ertd36e, NOT, Not56, Not56 like protein, NOT56L |
| Calculated Molecular Weight | 438 aa, 50 kDa |
| Observed Molecular Weight | 50-55 kDa |
| GenBank Accession Number | BC002839 |
| Gene Symbol | ALG3 |
| Gene ID (NCBI) | 10195 |
| RRID | AB_2878663 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92685 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALG3 antibody 20290-1-AP | Download protocol |
| IHC protocol for ALG3 antibody 20290-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Mol Biosci Expression of ALG3 in Hepatocellular Carcinoma and Its Clinical Implication. | ||
BMC Bioinformatics A novel glycosylation-related gene signature predicts survival in patients with lung adenocarcinoma | ||
J Exp Clin Cancer Res ALG3 contributes to stemness and radioresistance through regulating glycosylation of TGF-β receptor II in breast cancer. | ||
Heliyon Identification of ALG3 as a potential prognostic biomarker in lung adenocarcinoma
| ||
Metabolites Integrating TCGA and Single-Cell Sequencing Data for Hepatocellular Carcinoma: A Novel Glycosylation (GLY)/Tumor Microenvironment (TME) Classifier to Predict Prognosis and Immunotherapy Response | ||
Cells ALG3 Promotes Peritoneal Metastasis of Ovarian Cancer through Increasing Interaction of α1,3-mannosylated uPAR and ADAM8
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