验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, MCF-7 cells, human placenta tissue, mouse liver tissue |
| Positive IHC detected in | mouse placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ALG3 Ready-To-Use IHC Kit. ALG3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 1 publications below |
| IF | See 1 publications below |
产品信息
28084-1-AP targets ALG3 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | Human, Mouse |
| 文献引用反应性 | human |
| 免疫原 | ALG3 fusion protein Ag26382 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | asparagine-linked glycosylation 3, alpha-1,3- mannosyltransferase homolog (S. cerevisiae) |
| 别名 | ALG3, CDGS4, D16Ertd36e, NOT, Not56, Not56 like protein, NOT56L |
| 计算分子量 | 438 aa, 50 kDa |
| 观测分子量 | 50-60 kDa |
| GenBank蛋白编号 | BC002839 |
| 基因名称 | ALG3 |
| Gene ID (NCBI) | 10195 |
| RRID | AB_3669644 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q92685 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Alpha-1,3-mannosyltransferase (ALG3) encodes an endoplasmic reticulum-localized ALG3 family member that participates in N-glycan synthesis. ALG3 contributes to high mannose-type N-glycans in several cancers, and mannose-type N-glycan upregulation has been shown to be related to cancer progression. ALG3 is overexpressed in oral squamous cell carcinoma(PMID: 33084111), non-small cell lung cancer(PMID: 31899049), and breast cancer(PMID: 33931075) and hepatocellular carcinoma(PMID: 35782861).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALG3 antibody 28084-1-AP | Download protocol |
| IHC protocol for ALG3 antibody 28084-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |