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ALG3 Polyclonal antibody

ALG3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse and More (1)

Applications

WB, IHC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 28084-1-AP

Print datasheet

Synonyms

ALG3, CDGS4, D16Ertd36e, NOT, Not56, Not56 like protein, NOT56L

验证数据展示

  • WB analysis of HeLa using 28084-1-AP

    WB analysis of HeLa using 28084-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 28084-1-AP (ALG3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse placenta using 28084-1-AP

    IHC staining of mouse placenta using 28084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 28084-1-AP (ALG3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALG3 antibody included
  • IHC staining of mouse placenta using 28084-1-AP

    IHC staining of mouse placenta using 28084-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse placenta tissue slide using 28084-1-AP (ALG3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ALG3 antibody included

经过测试的应用

Positive WB detected inHeLa cells, MCF-7 cells, human placenta tissue, mouse liver tissue
Positive IHC detected inmouse placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ALG3 Ready-To-Use IHC Kit. ALG3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28084-1-AP targets ALG3 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Cited ApplicationsWB, IF
Tested Reactivity Human, Mouse
Cited Reactivityhuman
Immunogen ALG3 fusion protein Ag26382 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name asparagine-linked glycosylation 3, alpha-1,3- mannosyltransferase homolog (S. cerevisiae)
Synonyms ALG3, CDGS4, D16Ertd36e, NOT, Not56, Not56 like protein, NOT56L
Calculated Molecular Weight 438 aa, 50 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC002839
Gene Symbol ALG3
Gene ID (NCBI) 10195
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ92685
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Alpha-1,3-mannosyltransferase (ALG3) encodes an endoplasmic reticulum-localized ALG3 family member that participates in N-glycan synthesis. ALG3 contributes to high mannose-type N-glycans in several cancers, and mannose-type N-glycan upregulation has been shown to be related to cancer progression. ALG3 is overexpressed in oral squamous cell carcinoma(PMID: 33084111), non-small cell lung cancer(PMID: 31899049), and breast cancer(PMID: 33931075) and hepatocellular carcinoma(PMID: 35782861).

实验方案

Product Specific Protocols
WB protocol for ALG3 antibody 28084-1-APDownload protocol
IHC protocol for ALG3 antibody 28084-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB,IF

Comput Biol Med

Deep pan-cancer analysis and multi-omics evidence reveal that ALG3 inhibits CD8+ T cell infiltration by suppressing chemokine secretion and is associated with 5-fluorouracil sensitivity

Authors - Zhixuan Wu
View Article