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ALG5 Polyclonal antibody

ALG5 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 16046-1-AP

Print datasheet

Synonyms

HSPC149, EC:2.4.1.117, DolP-glucosyltransferase, Dolichyl-phosphate beta-glucosyltransferase, Asparagine-linked glycosylation protein 5 homolog

验证数据展示

  • WB analysis of human kidney using 16046-1-AP

    WB analysis of human kidney using 16046-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 16046-1-AP (ALG5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse liver using 16046-1-AP

    IHC staining of mouse liver using 16046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 16046-1-AP

    IHC staining of mouse liver using 16046-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16046-1-AP (ALG5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HEK-293 using 16046-1-AP

    IF Staining of HEK-293 using 16046-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 16046-1-AP (ALG5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inhuman kidney tissue, mouse liver tissue
Positive IHC detected inmouse liver tissue, human liver cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16046-1-AP targets ALG5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ALG5 fusion protein Ag8990 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name asparagine-linked glycosylation 5, dolichyl-phosphate beta-glucosyltransferase homolog (S. cerevisiae)
Synonyms HSPC149, EC:2.4.1.117, DolP-glucosyltransferase, Dolichyl-phosphate beta-glucosyltransferase, Asparagine-linked glycosylation protein 5 homolog
Calculated Molecular Weight 324 aa, 37 kDa
Observed Molecular Weight 37 kDa
GenBank Accession NumberBC012531
Gene Symbol ALG5
Gene ID (NCBI) 29880
RRIDAB_2273966
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y673
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dolichyl-phosphate beta-glucosyltransferase(ALG5), belongs to the family of glycosyltransferases, specifically the hexosyltransferases. ALG5 is widely expressed in pancreas, placenta, liver, heart, brain, kidney, skeletal muscle, and lung. ALG5 can catalyze the chemical reaction between UDP-glucose and UDP with dolichyl phosphate.

实验方案

Product Specific Protocols
WB protocol for ALG5 antibody 16046-1-APDownload protocol
IHC protocol for ALG5 antibody 16046-1-APDownload protocol
IF protocol for ALG5 antibody 16046-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols