验证数据展示
经过测试的应用
| Positive WB detected in | A549 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
产品信息
27973-1-AP targets ALKBH1 in WB, ELISA applications and shows reactivity with Human samples.
| 经测试应用 | WB, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | Human |
| 文献引用反应性 | human |
| 免疫原 | ALKBH1 fusion protein Ag27520 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | alkB, alkylation repair homolog 1 (E. coli) |
| 别名 | ABH, ABH1, alkB, ALKBH, ALKBH1, DNA lyase ABH1, hABH |
| 计算分子量 | 389 aa, 44 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC025787 |
| 基因名称 | ALKBH1 |
| Gene ID (NCBI) | 8846 |
| RRID | AB_2881025 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q13686 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Two RNA m6A demethylases, FTO and ALKBH5, have been identified since 2011, with both capable of removing the methyl group of m6A on poly-adenylated RNA through an iron-dependent oxidative demethylation mechanism. ALKBH1 as a RNA demethylase that mediates removal of the methyl group from N1-methyladenosine (m1A) in tRNA. ALKBH1 can tune translation initiation and elongation by regulating the cellular levels of tRNAiMet and the association of other ALKBH1 target tRNAs to polysomes, thereby affecting protein synthesis.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALKBH1 antibody 27973-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |