验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse kidney tissue, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human lung cancer tissue, human testis tissue, human prostate cancer tissue, human pancreas cancer tissue, rat stomach tissue, mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ALKBH3 Ready-To-Use IHC Kit. ALKBH3 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 3 publications below |
产品信息
12292-1-AP targets ALKBH3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 | ALKBH3 fusion protein Ag2982 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | alkB, alkylation repair homolog 3 (E. coli) |
| 别名 | ABH3, ALKBH3, DEPC 1, DEPC1, PCA1, Prostate cancer antigen 1 |
| 计算分子量 | 286 aa, 33 kDa |
| 观测分子量 | 22-25 kDa, 33kDa |
| GenBank蛋白编号 | BC015155 |
| 基因名称 | ALKBH3 |
| Gene ID (NCBI) | 221120 |
| RRID | AB_11125161 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96Q83 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALKBH3 antibody 12292-1-AP | Download protocol |
| IHC protocol for ALKBH3 antibody 12292-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Gastroenterol Hepatol ALKBH3 expression is associated with tumor recurrence and unfavorable prognosis in hepatocellular carcinoma.
| ||
Cell Biosci m1A demethylase Alkbh3 regulates neurogenesis through m1A demethylation of Mmp15 mRNA | ||
Virol J Evaluation of ALKBH2 and ALKBH3 gene regulation in patients with adult T-cell leukemia/lymphoma | ||
Biomedicines The Roles of AGTRAP, ALKBH3, DIVERSIN, NEDD8 and RRM1 in Glioblastoma Pathophysiology and Prognosis | ||
Clin Transl Med PUS7-dependent pseudouridylation of ALKBH3 mRNA inhibits gastric cancer progression |
