- Featured Product
- KD/KO Validated
ALKBH3 Polyclonal antibody
ALKBH3 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | Jurkat cells, mouse kidney tissue, mouse heart tissue, rat heart tissue |
| Positive IHC detected in | human lung cancer tissue, human testis tissue, human prostate cancer tissue, human pancreas cancer tissue, rat stomach tissue, mouse stomach tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ALKBH3 Ready-To-Use IHC Kit. ALKBH3 primary antibody included.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 3 publications below |
| IHC | See 3 publications below |
产品信息
12292-1-AP targets ALKBH3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB, IHC |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Immunogen | ALKBH3 fusion protein Ag2982 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | alkB, alkylation repair homolog 3 (E. coli) |
| Synonyms | ABH3, ALKBH3, DEPC 1, DEPC1, PCA1, Prostate cancer antigen 1 |
| Calculated Molecular Weight | 286 aa, 33 kDa |
| Observed Molecular Weight | 22-25 kDa, 33kDa |
| GenBank Accession Number | BC015155 |
| Gene Symbol | ALKBH3 |
| Gene ID (NCBI) | 221120 |
| RRID | AB_11125161 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96Q83 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALKBH3 antibody 12292-1-AP | Download protocol |
| IHC protocol for ALKBH3 antibody 12292-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Gastroenterol Hepatol ALKBH3 expression is associated with tumor recurrence and unfavorable prognosis in hepatocellular carcinoma.
| ||
Cell Biosci m1A demethylase Alkbh3 regulates neurogenesis through m1A demethylation of Mmp15 mRNA | ||
Virol J Evaluation of ALKBH2 and ALKBH3 gene regulation in patients with adult T-cell leukemia/lymphoma | ||
Biomedicines The Roles of AGTRAP, ALKBH3, DIVERSIN, NEDD8 and RRM1 in Glioblastoma Pathophysiology and Prognosis | ||
Clin Transl Med PUS7-dependent pseudouridylation of ALKBH3 mRNA inhibits gastric cancer progression |
