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验证数据展示

  • WB analysis using 84314-2-RR

    WB analysis using 84314-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84314-2-RR (ALKBH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84314-2-RR

    WB analysis using 84314-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84314-2-RR (ALKBH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 84314-2-RR

    FC experiment of HeLa using 84314-2-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ALKBH3 Recombinant antibody (84314-2-RR, Clone:241650F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of PC-3 using 84314-2-RR

    FC experiment of PC-3 using 84314-2-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug ALKBH3 Recombinant antibody (84314-2-RR, Clone:241650F10) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84314-2-RR

    Affinity and Kinetic Characterization of 84314-2-RR

    Biolayer interferometry (BLl) kinetic assays of 84314-2-RR against Human ALKBH3 were performed. The affinity constant is 1.99 nM.

ALKBH3 Recombinant antibody

ALKBH3 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 84314-2-RR

产品说明书

CloneNo. 241650F10

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, FC (Intra), ELISA

别名

DEPC-1, DEPC1, DEPC 1, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, ABH3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA375 cells, mouse kidney tissue, SGC-7901 cells, mouse heart tissue, rat heart tissue
Positive FC (Intra) detected inPC-3 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy ALKBH3 Ready-To-Use IHC Kit. ALKBH3 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84314-2-RR targets ALKBH3 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ALKBH3 fusion protein Ag2982 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 alkB, alkylation repair homolog 3 (E. coli)
别名 DEPC-1, DEPC1, DEPC 1, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, ABH3
计算分子量 286 aa, 33 kDa
观测分子量30-33 kDa
GenBank蛋白编号BC015155
基因名称 ALKBH3
Gene ID (NCBI) 221120
RRIDAB_3671855
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ96Q83
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.

实验方案

Product Specific Protocols
WB protocol for ALKBH3 antibody 84314-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols