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ALKBH3 Recombinant antibody

ALKBH3 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241650C3

Cat no : 84314-3-RR

Print datasheet

Synonyms

DEPC-1, DEPC1, DEPC 1, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, ABH3

验证数据展示

  • WB analysis using 84314-3-RR

    WB analysis using 84314-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84314-3-RR (ALKBH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84314-3-RR

    WB analysis using 84314-3-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84314-3-RR (ALKBH3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 84314-3-RR

    IF Staining of A431 using 84314-3-RR

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using ALKBH3 antibody (84314-3-RR, Clone: 241650C3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • FC experiment of HeLa using 84314-3-RR

    FC experiment of HeLa using 84314-3-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug ALKBH3 Recombinant antibody (84314-3-RR, Clone:241650C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of PC-3 using 84314-3-RR

    FC experiment of PC-3 using 84314-3-RR

    1x10^6 PC-3 cells were intracellularly stained with 0.25 ug ALKBH3 Recombinant antibody (84314-3-RR, Clone:241650C3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 84314-3-RR

    Affinity and Kinetic Characterization of 84314-3-RR

    Biolayer interferometry (BLl) kinetic assays of 84314-3-RR against Human ALKBH3 were performed. The affinity constant is 1.42 nM.

经过测试的应用

Positive WB detected inJurkat cells, mouse kidney tissue, mouse heart tissue, rat heart tissue
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inPC-3 cells, HeLa cells
Planning an IHC experiment? We recommend our IHCeasy ALKBH3 Ready-To-Use IHC Kit. ALKBH3 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

The immunogen of 84314-3-RR is ALKBH3 Fusion Protein expressed in E. coli.

Tested Applications WB, IF/ICC, FC (Intra), ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen ALKBH3 fusion protein Ag2982 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name alkB, alkylation repair homolog 3 (E. coli)
Synonyms DEPC-1, DEPC1, DEPC 1, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 3, ABH3
Calculated Molecular Weight 286 aa, 33 kDa
Observed Molecular Weight19-25 kDa
GenBank Accession NumberBC015155
Gene Symbol ALKBH3
Gene ID (NCBI) 221120
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ96Q83
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALKBH3, also named as ABH3 and DEPC-1, is a dioxygenase that mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It has a strong preference for single-stranded. ALKBH3 is requires molecular oxygen, alpha-ketoglutarate and iron. This gene has some isoforms with MW 19-25 kDa and 33 kDa.

实验方案

Product Specific Protocols
WB protocol for ALKBH3 antibody 84314-3-RRDownload protocol
IF protocol for ALKBH3 antibody 84314-3-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols