• Featured Product
  • KD/KO验证

ALKBH5 Polyclonal antibody

ALKBH5 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 16837-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat and More (3)

应用

WB, IHC, ELISA and More (3)

RNA demethylase ALKBH5, EC:1.14.11.53, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, ALKBH 5

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, HEK-293 cells, mouse brain tissue, rat brain tissue
Positive IHC detected inhuman testis tissue, human lung cancer tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ALKBH5 Ready-To-Use IHC Kit. ALKBH5 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

16837-1-AP targets ALKBH5 in WB, IHC, IF, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IHC, IF, CoIP, RIP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat, pig, chicken, bovine
免疫原 ALKBH5 fusion protein Ag10516 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 alkB, alkylation repair homolog 5 (E. coli)
别名 RNA demethylase ALKBH5, EC:1.14.11.53, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, ALKBH 5
计算分子量 458 aa, 51 kDa
观测分子量 40-50 kDa
GenBank蛋白编号BC062339
基因名称 ALKBH5
Gene ID (NCBI) 54890
RRIDAB_2242665
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6P6C2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.

实验方案

Product Specific Protocols
WB protocol for ALKBH5 antibody 16837-1-APDownload protocol
IHC protocol for ALKBH5 antibody 16837-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Genet

N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2.

Authors - Yuan Li
humanIF

Cell Res

The m6A methylome of SARS-CoV-2 in host cells.

Authors - Jun'e Liu
  • KD Validated
mouseWB,RIP

Nat Cell Biol

The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA.

Authors - Xiang-Qian Gao
humanWB

Nat Commun

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis

Authors - Jian Zhong
human,mouseWB

Nat Commun

Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma

Authors - Weiwei Dai
mouseWB

Nat Commun

ALKBH5-mediated m6A modification of IL-11 drives macrophage-to-myofibroblast transition and pathological cardiac fibrosis in mice

Authors - Tao Zhuang
Loading...