验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, HEK-293 cells, mouse brain tissue, rat brain tissue |
Positive IHC detected in | human testis tissue, human lung cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:2000-1:10000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
16837-1-AP targets ALKBH5 in WB, IHC, IF, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IF, CoIP, RIP |
经测试反应性 | human, mouse, rat |
文献引用反应性 | human, mouse, rat, pig, chicken, bovine |
免疫原 | ALKBH5 fusion protein Ag10516 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | alkB, alkylation repair homolog 5 (E. coli) |
别名 | RNA demethylase ALKBH5, EC:1.14.11.53, Alpha-ketoglutarate-dependent dioxygenase alkB homolog 5, ALKBH 5 |
计算分子量 | 458 aa, 51 kDa |
观测分子量 | 40-50 kDa |
GenBank蛋白编号 | BC062339 |
基因名称 | ALKBH5 |
Gene ID (NCBI) | 54890 |
RRID | AB_2242665 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q6P6C2 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ALKBH5, also named as ABH5 and OFOXD1, is a Dioxygenase that demethylates RNA by oxidative demethylation. It is a mammalian demethylase that oxidatively reverses m6A in mRNA in vitro and in vivo. ALKBH5 is required for spermatogenesis. There're some isoforms of ALKBH5 with MW 40-44 kDa and 52 kDa.
实验方案
Product Specific Protocols | |
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WB protocol for ALKBH5 antibody 16837-1-AP | Download protocol |
IHC protocol for ALKBH5 antibody 16837-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
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Nat Genet N6-Methyladenosine co-transcriptionally directs the demethylation of histone H3K9me2. | ||
Nat Cell Biol The piRNA CHAPIR regulates cardiac hypertrophy by controlling METTL3-dependent N6-methyladenosine methylation of Parp10 mRNA. | ||
Nat Commun Overcoming therapeutic resistance in oncolytic herpes virotherapy by targeting IGF2BP3-induced NETosis in malignant glioma | ||
Nat Commun ALKBH5-mediated m6A modification of IL-11 drives macrophage-to-myofibroblast transition and pathological cardiac fibrosis in mice |