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ALKBH7 Polyclonal antibody
ALKBH7 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | human testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
产品信息
15470-1-AP targets ALKBH7 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Applications | WB, IHC, ELISA Application Description |
| Cited Applications | WB |
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | ALKBH7 fusion protein Ag7772 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | alkB, alkylation repair homolog 7 (E. coli) |
| Synonyms | ABH7, ALKBH7, SPATA11, UNQ6002 |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC004393 |
| Gene Symbol | ALKBH7 |
| Gene ID (NCBI) | 84266 |
| RRID | AB_2226855 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BT30 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ALKBH7(Alkylated DNA repair protein alkB homolog 7) is also named as ABH7, SPATA11 and belongs to the alkB family. This protein may be a dioxygenase that requires molecular oxygen, alpha-ketoglutarate and iron. Quantitative real-time PCR detectes variable ALKBH7 expression in all 16 tissues examined, with highest expression in pancreas, followed by spleen, prostate, ovary, and placenta(PMID:17979886). The gene encodes a 25 kDa protein with a 20 amino acid signal peptide.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ALKBH7 antibody 15470-1-AP | Download protocol |
| IHC protocol for ALKBH7 antibody 15470-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol ALKBH7-mediated demethylation regulates mitochondrial polycistronic RNA processing.
| ||
Cell Death Dis ALKBH7 drives a tissue and sex-specific necrotic cell death response following alkylation-induced damage.
| ||
