ALOX15B Polyclonal antibody

ALOX15B Polyclonal Antibody for WB, ELISA
Cat No. 13073-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human and More (2)

应用

WB, ELISA and More (1)

15 lipoxygenase 2, 15-LOX-2, 15-lipoxygenase 2, 15 LOX B, 15 LOX 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, HEK-293 cells, HL-60 cells, HeLa cells, MCF-7 cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

13073-1-AP targets ALOX15B in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
文献引用应用WB, IHC
经测试反应性 human
文献引用反应性human, rat, pig
免疫原 ALOX15B fusion protein Ag3741 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 arachidonate 15-lipoxygenase, type B
别名 15 lipoxygenase 2, 15-LOX-2, 15-lipoxygenase 2, 15 LOX B, 15 LOX 2
计算分子量 676 aa, 76 kDa
观测分子量 67-76 kDa
GenBank蛋白编号BC035217
基因名称 ALOX15B
Gene ID (NCBI) 247
RRIDAB_2227031
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO15296
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

In humans, there are 6 functional lipoxygenases genes. Two of them encode for arachidonic acid 12-lipoxygenating (ALOX12, ALOX12B) and two for 15-lipoxygenating enzymes (ALOX15, ALOX15B). ALOX15B has been described as the most abundant 15-lipoxygenating enzyme species in human carotid atherosclerotic lesions and to be associated with cerebrovascular symptoms. (PMID:24373925). ALOX15B has 4 isoforms produced by alternative splicing with the MW of 76, 67, 69 and 73 kDa. 13073-1-AP can also recognize the mouse Alox8 protein.

实验方案

Product Specific Protocols
WB protocol for ALOX15B antibody 13073-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Nutrition

Intermittent fasting ameliorates neuronal ferroptosis and cognitive impairment in mice after traumatic brain injury

Authors - Qiuyun Yang
ratIHC

Exp Cell Res

Lipoxin A4 and its analog attenuate high fat diet-induced atherosclerosis via Keap1/Nrf2 pathway.

Authors - Fen Xu
WB

J Chem Neuroanat

Proanthocyanidin promotes functional recovery of spinal cord injury via inhibiting ferroptosis.

Authors - Huangao Zhou
pigWB

Theriogenology

The role of ALOX15B in heat stress-induced apoptosis of porcine sertoli cells.

Authors - Hongyan Xue
humanWB

Redox Biol

Excessive SOX8 reprograms energy and iron metabolism to prime hepatocellular carcinoma for ferroptosis

Authors - Xue Yang
humanWB

Lipids Health Dis

Lipid peroxidation-induced ferroptosis as a therapeutic target for mitigating neuronal injury and inflammation in sepsis-associated encephalopathy: insights into the hippocampal PEBP-1/15-LOX/GPX4 pathway

Authors - Haosen Wang
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