验证数据展示
经过测试的应用
| Positive WB detected in | human placenta tissue, mouse liver tissue, HepG2 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human placenta tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy ALPP/PLAP Ready-To-Use IHC Kit. ALPP/PLAP primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 9 publications below |
| IHC | See 3 publications below |
产品信息
18507-1-AP targets PLAP in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IP, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 | PLAP fusion protein Ag13361 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | alkaline phosphatase, placental (Regan isozyme) |
| 别名 | ALP, ALPP, PALP, Placental alkaline phosphatase, PLAP, PLAP 1 |
| 计算分子量 | 58 kDa |
| 观测分子量 | 66 kDa |
| GenBank蛋白编号 | BC009647 |
| 基因名称 | PLAP |
| Gene ID (NCBI) | 250 |
| RRID | AB_10644334 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P05187 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Placental alkaline phosphatase (PLAP) is a glycosyl phosphatidylinositol (GPI)-anchored sialoglycoprotein , which shows greater resistance to heat inactivation than other alkaline phosphatase. Presented at high levels in placental trophoblasts, PLAP is recognized as an important marker of differentiation in human malignancies.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for PLAP antibody 18507-1-AP | Download protocol |
| IHC protocol for PLAP antibody 18507-1-AP | Download protocol |
| IP protocol for PLAP antibody 18507-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
J Nanobiotechnology Ultrasound-triggered piezoelectric polyetheretherketone with boosted osteogenesis via regulating Akt/GSK3β/β-catenin pathway | ||
Aging (Albany NY) Primary cilia regulate gastric cancer-induced bone loss via cilia/Wnt/β-catenin signaling pathway. | ||
ACS Biomater Sci Eng 6-Bromoindirubin-3'-oxime Promotes Osteogenic Differentiation of Periodontal Ligament Stem Cells and Facilitates Bone Regeneration in a Mouse Periodontitis Model. | ||
Mol Cell Endocrinol Long noncoding RNA ZFAS1 suppresses osteogenic differentiation of bone marrow-derived mesenchymal stem cells by upregulating miR-499-EPHA5 axis. | ||
Amino Acids New aspects of antiproliferative activity of 4-hydroxybenzyl isothiocyanate, a natural H2S-donor. |
