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AMBN Polyclonal antibody

AMBN Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 26501-1-AP

Print datasheet

Synonyms

Ameloblastin

验证数据展示

  • WB analysis using 26501-1-AP

    WB analysis using 26501-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26501-1-AP (AMBN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IF Staining of HEK-293 using 26501-1-AP

    IF Staining of HEK-293 using 26501-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using AMBN antibody (26501-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IF/ICC detected inHEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26501-1-AP targets AMBN in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human
Immunogen AMBN fusion protein Ag24129 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name ameloblastin (enamel matrix protein)
Synonyms Ameloblastin
Calculated Molecular Weight 447 aa, 48 kDa
Observed Molecular Weight48-55 kDa
GenBank Accession NumberBC106931
Gene Symbol AMBN
Gene ID (NCBI) 258
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NP70
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMBN(Ameloblastin), the gene encodes the nonamelogenin enamel matrix protein ameloblastin. The encoded protein may be important in enamel matrix formation and mineralization. Mutations in this gene may be associated with dentinogenesis imperfect and autosomal dominant amylogenesis imperfect. It is expected to be located in the Vesicles, in addition localized to the Nucleoplasm, which is enriched in brain, and located at the Tomes processes of secretory ameloblasts and in the sheath space between rod-interrod enamel. The molecular weight of AMBN is 48 kDa. It also has phosphorylation modification.

实验方案

Product Specific Protocols
WB protocol for AMBN antibody 26501-1-APDownload protocol
IF protocol for AMBN antibody 26501-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols