验证数据展示
经过测试的应用
Positive WB detected in | A431 cells |
Positive IHC detected in | human prostate cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
11052-1-AP targets AMD1 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
经测试应用 | WB, IHC, ELISA Application Description |
文献引用应用 | WB, IHC, IF |
经测试反应性 | human |
文献引用反应性 | human, mouse |
免疫原 | AMD1 fusion protein Ag1525 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | adenosylmethionine decarboxylase 1 |
别名 | ADOMETDC, AMD, AMD1, SAMDC |
计算分子量 | 38 kDa, 32 kDa |
观测分子量 | 38-40 kDa |
GenBank蛋白编号 | BC000171 |
基因名称 | AMD1 |
Gene ID (NCBI) | 262 |
RRID | AB_2226430 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | P17707 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
Product Specific Protocols | |
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WB protocol for AMD1 antibody 11052-1-AP | Download protocol |
IHC protocol for AMD1 antibody 11052-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Nature mTORC1-dependent AMD1 regulation sustains polyamine metabolism in prostate cancer.
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Nucleic Acids Res Genome-wide RNAi screen identifies novel players in human 60S subunit biogenesis including key enzymes of polyamine metabolism. | ||
Clin Transl Med AMD1 upregulates hepatocellular carcinoma cells stemness by FTO mediated mRNA demethylation.
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Breast Cancer Res AMD1 promotes breast cancer aggressiveness via a spermidine-eIF5A hypusination-TCF4 axis | ||
iScience The KRAS-G12D mutation induces metabolic vulnerability in B-cell acute lymphoblastic leukemia. | ||
iScience Linkage of methionine addiction, histone lysine hypermethylation, and malignancy. |