验证数据展示
经过测试的应用
Positive WB detected in | mouse skeletal muscle tissue |
Positive IHC detected in | mouse colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
21171-1-AP targets AMDHD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human, mouse |
Immunogen | AMDHD1 fusion protein Ag15420 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | amidohydrolase domain containing 1 |
Synonyms | AMDHD1, HMFT1272, Probable imidazolonepropionase |
Calculated Molecular Weight | 426 aa, 47 kDa |
Observed Molecular Weight | 50 kDa |
GenBank Accession Number | BC029146 |
Gene Symbol | AMDHD1 |
Gene ID (NCBI) | 144193 |
RRID | AB_3085642 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q96NU7 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
AMDHD1 is a protein-coding gene that encodes the enzyme imidazolonepropionase. It is involved in the histidine catabolic process and has one potential isoform. The protein of AMDHD1 is located in the cytoplasm, and is expressed in all tissues, mainly in the liver, kidney and adrenal gland.
实验方案
Product Specific Protocols | |
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WB protocol for AMDHD1 antibody 21171-1-AP | Download protocol |
IHC protocol for AMDHD1 antibody 21171-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |