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验证数据展示

  • WB analysis of mouse skeletal muscle using 21171-1-AP

    WB analysis of mouse skeletal muscle using 21171-1-AP

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 21171-1-AP (AMDHD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 21171-1-AP

    IHC staining of mouse colon using 21171-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21171-1-AP (AMDHD1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse colon using 21171-1-AP

    IHC staining of mouse colon using 21171-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 21171-1-AP (AMDHD1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

AMDHD1 Polyclonal antibody

AMDHD1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 21171-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

AMDHD1, HMFT1272, Probable imidazolonepropionase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inmouse skeletal muscle tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

21171-1-AP targets AMDHD1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen AMDHD1 fusion protein Ag15420 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name amidohydrolase domain containing 1
Synonyms AMDHD1, HMFT1272, Probable imidazolonepropionase
Calculated Molecular Weight 426 aa, 47 kDa
Observed Molecular Weight50 kDa
GenBank Accession NumberBC029146
Gene Symbol AMDHD1
Gene ID (NCBI) 144193
RRIDAB_3085642
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96NU7
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

AMDHD1 is a protein-coding gene that encodes the enzyme imidazolonepropionase. It is involved in the histidine catabolic process and has one potential isoform. The protein of AMDHD1 is located in the cytoplasm, and is expressed in all tissues, mainly in the liver, kidney and adrenal gland.

实验方案

Product Specific Protocols
WB protocol for AMDHD1 antibody 21171-1-APDownload protocol
IHC protocol for AMDHD1 antibody 21171-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols