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AMFR/GP78 Polyclonal antibody

AMFR/GP78 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (2)

Conjugate

Unconjugated

Publications

Cat no : 16675-1-AP

Print datasheet

Synonyms

AMF receptor, isoform 2, AMFR, GP78, RING finger protein 45, RNF45

验证数据展示

WB analysis of HepG2 using 16675-1-AP
WB result of AMFR antibody (16675-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-AMFR transfected HepG2 cells.
WB analysis using 16675-1-AP
HepG2 lysates were subjected to SDS PAGE followed by western blot with 16675-1-AP (AMFR antibody) at dilution of 1:4000 incubated at 37℃ and 100℃ for 1.5 hours.
IP experiment of MCF-7 using 16675-1-AP
IP result of anti-AMFR/GP78 (IP:16675-1-AP, 4ug; Detection:16675-1-AP 1:300) with MCF-7 cells lysate 2400 ug.
IHC staining of mouse ovary using 16675-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16675-1-AP (AMFR/GP78 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse ovary using 16675-1-AP
Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 16675-1-AP (AMFR/GP78 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of HepG2 using 16675-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human AMFR/GP78 (16675-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).
IF Staining of HepG2 using 16675-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using AMFR/GP78 antibody (16675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Cat No. 16675-1-AP

Size

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经过测试的应用

Positive WB detected in	37°C incubated HepG2 cells, HepG2 cells, MCF-7 cells
Positive IP detected in	MCF-7 cells
Positive IHC detected in	mouse ovary tissue, human kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells
Positive FC detected in	HepG2 cells

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)	FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 9 publications below
WB	See 29 publications below
IHC	See 1 publications below
IF	See 5 publications below
IP	See 1 publications below
CoIP	See 2 publications below

产品信息

16675-1-AP targets AMFR/GP78 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IHC, IF, IP, CoIP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	AMFR/GP78 fusion protein Ag10178 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	autocrine motility factor receptor
Synonyms	AMF receptor, isoform 2, AMFR, GP78, RING finger protein 45, RNF45
Calculated Molecular Weight	643 aa, 73 kDa
Observed Molecular Weight	73 kDa
GenBank Accession Number	BC069197
Gene Symbol	AMFR/GP78
Gene ID (NCBI)	267
RRID	AB_2226463
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UKV5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

AMFR(Autocrine motility factor receptor) is also named as RNF45,gp78.It is the receptor of Autocrine motility factor(AMF),which is a protein secreted by tumor cells that stimulates tumor motility. AMFR has a potential N-glycosylation site, and several potential O-glycosylation sites(PMID:10456327).In normal fibroblasts,the expression of AMFR appeared to be regulated by cell-cell contact(PMID:7626106).

实验方案

Product Specific Protocols
WB protocol for AMFR/GP78 antibody 16675-1-AP	Download protocol
IHC protocol for AMFR/GP78 antibody 16675-1-AP	Download protocol
IF protocol for AMFR/GP78 antibody 16675-1-AP	Download protocol
IP protocol for AMFR/GP78 antibody 16675-1-AP	Download protocol
FC protocol for AMFR/GP78 antibody 16675-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Cell BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation. Authors - Fabien Llambi KD Validated View Article
human, mouse	WB, IF	Immunity The E3 Ubiquitin Ligase AMFR and INSIG1 Bridge the Activation of TBK1 Kinase by Modifying the Adaptor STING. Authors - Qiang Wang KD Validated View Article
human	IF	PLoS Biol Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules. Authors - Guang Gao View Article
mouse	WB	Brief Bioinform Standigm ASK™: knowledge graph and artificial intelligence platform applied to target discovery in idiopathic pulmonary fibrosis Authors - Seokjin Han View Article
human	WB	EMBO Rep MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins. Authors - Sandra Stefanovic-Barrett KO Validated View Article
human	WB	Elife Aberrant corticosteroid metabolism in tumor cells enables GR takeover in enzalutamide resistant prostate cancer. Authors - Jianneng Li KD Validated View Article

AMFR/GP78 Polyclonal antibody

AMFR/GP78 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

验证数据展示

WB analysis of HepG2 using 16675-1-AP

WB analysis using 16675-1-AP

IP experiment of MCF-7 using 16675-1-AP

IHC staining of mouse ovary using 16675-1-AP

IHC staining of mouse ovary using 16675-1-AP

FC experiment of HepG2 using 16675-1-AP

IF Staining of HepG2 using 16675-1-AP

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

BOK Is a Non-canonical BCL-2 Family Effector of Apoptosis Regulated by ER-Associated Degradation.

The E3 Ubiquitin Ligase AMFR and INSIG1 Bridge the Activation of TBK1 Kinase by Modifying the Adaptor STING.

Reticulon and CLIMP-63 regulate nanodomain organization of peripheral ER tubules.

Standigm ASK™: knowledge graph and artificial intelligence platform applied to target discovery in idiopathic pulmonary fibrosis

MARCH6 and TRC8 facilitate the quality control of cytosolic and tail-anchored proteins.

Aberrant corticosteroid metabolism in tumor cells enables GR takeover in enzalutamide resistant prostate cancer.

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Flow Cytometry Perm Buffer (10X)