验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, mouse ovary tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
84153-5-RR targets AMH in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | AMH fusion protein Ag5670 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | anti-Mullerian hormone |
| 别名 | Muellerian inhibiting factor, Anti-Muellerian hormone (AMH), Anti-Muellerian hormone, anti Mullerian hormone, Anti muellerian hormone |
| 计算分子量 | 59 kDa |
| 观测分子量 | 60-65 kDa |
| GenBank蛋白编号 | BC049194 |
| 基因名称 | AMH |
| Gene ID (NCBI) | 268 |
| RRID | AB_3671717 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P03971 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Anti-Müllerian hormone (AMH), also called Müllerian-inhibiting substance (MIS), is a hormone that is best known for its production by fetal testes in mammals and as the inhibitor of Müllerian duct development in males. AMH is also expressed in granulosa cells of preantral and small antral follicles in the ovary. More recently, AMH has been studied for its role in ovarian folliculogenesis and as a potential marker of ovarian reserve. The deduced protein sequence of the AMH monomer contains a 25 amino acid secretion specific signal peptide and a monomeric protein of 535 amino acids that, when glycosylated at two glycosylation sites, has a molecular weight of 65-70 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for AMH antibody 84153-5-RR | Download protocol |
| IHC protocol for AMH antibody 84153-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |