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AMN1 Polyclonal antibody

AMN1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14694-1-AP

Print datasheet

Synonyms

AMN1, Protein AMN1 homolog

验证数据展示

  • WB analysis of human colon using 14694-1-AP

    WB analysis of human colon using 14694-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 14694-1-AP (AMN1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 14694-1-AP

    IHC staining of human colon cancer using 14694-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 14694-1-AP (AMN1 antibody) at dilution of 1:100 (under 10x lens).

经过测试的应用

Positive WB detected inhuman colon tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

14694-1-AP targets AMN1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen AMN1 fusion protein Ag6257 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name antagonist of mitotic exit network 1 homolog (S. cerevisiae)
Synonyms AMN1, Protein AMN1 homolog
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC067906
Gene Symbol AMN1
Gene ID (NCBI) 196394
RRIDAB_2226647
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8IY45
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for AMN1 antibody 14694-1-APDownload protocol
IHC protocol for AMN1 antibody 14694-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols